Statement of problem: Congenital Diaphragmatic Hernia (CDH) is a fatal disease associated with abnormal lung development. Our aim is to establish a clinical database coupled with a biobank for CDH patients to facilitate further research. Methods: Our database and tissue bank were formed from the Pathology autopsy database for the CDH non-survivors and control subjects. We compared our database with CDH survivors records from a separate REDCap clinical CDH database. Results: The study comprised a total of 155 subjects, of which, 99 were CDH survivors, 40 were CDH non-survivors, and 16 were deceased subjects without CDH used as controls. Seventeen lung tissue blocks with different stages of lung development were obtained. Male subjects (59.7%) and left-sided CDH (76.9%) were more common. Among non-survivors we found a lower lung-to-body-weight (mean value 0.008 ± 0.004) compared to controls (0.019 ± 0.006). Conclusion: This clinical database and tissue bank can help future research.