Cytotoxic effects of pyocin S2 produced by Pseudomonas aeruginosa on the growth of three human cell lines

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dc.contributor.authorAbdi-Ali, A
dc.contributor.authorWorobec, EA
dc.contributor.authorDeezagi, A
dc.contributor.authorMalekzadeh, F
dc.date.accessioned2007-10-10T18:07:17Z
dc.date.available2007-10-10T18:07:17Z
dc.date.issued2004-05-31
dc.description.abstractPyocin typing of 82 Pseudomonas aeruginosa strains, collected from different Iranian clinical sources, revealed that one isolate, P. aeruginosa 42A, produced pyocin S2, a protease-sensitive bacteriocin. Pyocin S2 production was induced by mitomycin C (2 mug/mL) in the pyocin S2 producer P. aeruginosa 42A. Pyocin S2 was purified using ion exchange chromatography with CM-Sepharose CL-6B and sodium phosphate buffer (pH 8) from an 80% ammonium sulfate precipitate of whole-cell lysates. Pyocin activity of the fractions was detected using the Govan spot testing method. The purity of the active fraction was confirmed by SDS-PAGE, where a single band with a molecular mass of 74 kDa was detected. Cytotoxic effects of purified pyocin S2 and partially purified pyocin from P. aeruginosa 42A on the human tumor cell lines HepG2 and Im9 and the normal human cell line HFFF (Human Foetal Foreskin Fibroblast) were studied by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The results demonstrated that partially purified pyocin and pyocin S2 exhibited substantial inhibitory effects on the growth of the tumor cell lines HepG2 and Im9, while no inhibitory effects were observed on the normal cell line HFFF. Pure lipopolysaccharide was used as a control and was found to have no inhibitory effect on any of the cell lines tested.en
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dc.format.mimetypeapplication/pdf
dc.identifier.citation0008-4166; CAN J MICROBIOL, MAY 2004, vol. 50, no. 5, p.375 to 381.en
dc.identifier.doihttp://dx.doi.org/10.1139/W04-019
dc.identifier.urihttp://hdl.handle.net/1993/2927
dc.language.isoengen_US
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dc.rightsopen accessen_US
dc.statusPeer revieweden
dc.subjectPseudomonas aeruginosaen
dc.subjectpyocinen
dc.subjectcytotoxicityen
dc.subjectMTT assayen
dc.subjectTRANSFERRIN RECEPTORSen
dc.subjectCOLORIMETRIC ASSAYen
dc.subjectPURIFICATIONen
dc.subjectBACTERIOCINen
dc.subjectCOLICINen
dc.subjectNEOPLASIAen
dc.subjectPROTEINSen
dc.subjectGENESen
dc.subjectVITROen
dc.subjectPAO1en
dc.titleCytotoxic effects of pyocin S2 produced by Pseudomonas aeruginosa on the growth of three human cell linesen
dc.typejournal articleen_US
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