The intestinal helminths of Perca flavescens (Mitchill) and Stizostedion vitreum vitreum (Mitchill) are recorded. Twelve species of helminths, five cestodes, three trematodes, three nematodes, and one acanthocephalan are reported from eighty fish examined. No new species are described. A number of new locality and host records are reported. Triaenophorus crassus infestation in Salvelinus namaycush (Walbaum) is recorded. One hundred and two Salvelinus namaycush were examined for cysts of Triaenophorus crassus. Unencysted plerocercoids of Triaenophorus crassus in Salvelinus namaycush are reported. Synoptic tables and maps of distribution of parasites reported from fishes in Manitoba are included. A note on host-parasite relationships and ecology of the parasite fauna of Perca flavescens and Stizostedion vitreum vitreum is presented.