A novel role of the vaccinia virus mRNA capping enzyme in determining virus host range
Loading...
Date
2018-12-20
Authors
Varga, Jessie
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
E3 family proteins are a poxvirus defense gene that have a significant role in host range determination. Their biological function has largely been attributed to the ability of the protein to bind double-stranded RNA. Increasing evidence indicates this mechanism is more complex. For example, the ortholog, SPPV034, is unable to rescue replication of a recombinant virus even though it retains the ability to bind dsRNA.
The Vaccinia virus capping enzyme (the D1-D12 heterodimer) is a cofactor for E3 protein in determining host range. A D1-G801S mutation rescued replication of the SPPV034 recombinant virus and inhibited activation of host responses. A D12-S131N partially rescue replication, but could not fully antagonize host responses. These results indicate that E3 proteins operate on a threshold mechanism determined by intrinsic properties. The D1-D12 heterodimer mutations altered the quantity of dsRNA produced during infections so that the upper limit of the SPPV034 protein was not reached.
Description
Keywords
Vaccinia Virus