A novel role of the vaccinia virus mRNA capping enzyme in determining virus host range
| dc.contributor.author | Varga, Jessie | |
| dc.contributor.examiningcommittee | Booth, Stephanie (Medical Microbiology and Infectious Diseases) Kung, Sam (Immunology) | en_US |
| dc.contributor.supervisor | Cao, Jingxin (Medical Microbiology and Infectious Diseases) | en_US |
| dc.date.accessioned | 2019-01-08T18:38:59Z | |
| dc.date.available | 2019-01-08T18:38:59Z | |
| dc.date.issued | 2018-12-20 | en_US |
| dc.date.submitted | 2018-12-20T18:18:15Z | en |
| dc.date.submitted | 2019-01-08T16:02:42Z | en |
| dc.degree.discipline | Medical Microbiology and Infectious Diseases | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | E3 family proteins are a poxvirus defense gene that have a significant role in host range determination. Their biological function has largely been attributed to the ability of the protein to bind double-stranded RNA. Increasing evidence indicates this mechanism is more complex. For example, the ortholog, SPPV034, is unable to rescue replication of a recombinant virus even though it retains the ability to bind dsRNA. The Vaccinia virus capping enzyme (the D1-D12 heterodimer) is a cofactor for E3 protein in determining host range. A D1-G801S mutation rescued replication of the SPPV034 recombinant virus and inhibited activation of host responses. A D12-S131N partially rescue replication, but could not fully antagonize host responses. These results indicate that E3 proteins operate on a threshold mechanism determined by intrinsic properties. The D1-D12 heterodimer mutations altered the quantity of dsRNA produced during infections so that the upper limit of the SPPV034 protein was not reached. | en_US |
| dc.description.note | February 2019 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/33660 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Vaccinia Virus | en_US |
| dc.title | A novel role of the vaccinia virus mRNA capping enzyme in determining virus host range | en_US |
| dc.type | master thesis | en_US |