Layer-by-layer assembly of epidermal growth factors on polyurethane films for wound closure
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Date
2014-02-13
Authors
Kulkarni, Abhilash
Diehl-Jones, William
Ghanbar, Sadegh
Liu, Song
Journal Title
Journal ISSN
Volume Title
Publisher
SAGE Publishing
Abstract
To facilitate the healing of chronic wounds, growth factors such as epidermal growth factor need to be safely encapsulated
for their sustained and effective delivery to the wound bed. Using a layer-by-layer assembly technique, epidermal
growth factor is successfully encapsulated on the surface of poly(acrylic acid)-modified polyurethane film. The amount of
encapsulated epidermal growth factor is controlled by adjusting the number of chitosan/epidermal growth factor bilayers.
A controlled release of epidermal growth factor from the surface of polyurethane film for a period of five days is achieved
with well-retained bioactivity (over 90%) as evidenced by a cell proliferation assay. In an in vitro cellular wounding assay,
the cell gap covered with the epidermal growth factor-loaded polyurethane film closes at a rate more than twice as fast as
that covered with a control polyurethane film. Fluorescent staining of F-actin reveals that the released epidermal growth
factor induces differences in cytoskeletal organization, suggesting that stimulated cell migration also contributes to the
close of the cell gap.
Description
Keywords
Delivery of epidermal growth factor, Layer-by-Layer assembly, Wound healing, Polyurethane film
Citation
Kulkarni, A.; Diehl-Jones, W.; Liu, S.* Layer-by-Layer assembly of epidermal growth factors on polyurethane films for wound closure. Journal of Biomaterials Applications, 2014; 29(2): 278-290.