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dc.contributor.author Kulkarni, Abhilash
dc.contributor.author Diehl-Jones, William
dc.contributor.author Ghanbar, Sadegh
dc.contributor.author Liu, Song
dc.date.accessioned 2018-10-09T14:45:45Z
dc.date.available 2018-10-09T14:45:45Z
dc.date.issued 2014-02-13
dc.date.submitted 2018-10-08T00:40:33Z en
dc.identifier.citation Kulkarni, A.; Diehl-Jones, W.; Liu, S.* Layer-by-Layer assembly of epidermal growth factors on polyurethane films for wound closure. Journal of Biomaterials Applications, 2014; 29(2): 278-290. en_US
dc.identifier.uri http://hdl.handle.net/1993/33528
dc.description.abstract To facilitate the healing of chronic wounds, growth factors such as epidermal growth factor need to be safely encapsulated for their sustained and effective delivery to the wound bed. Using a layer-by-layer assembly technique, epidermal growth factor is successfully encapsulated on the surface of poly(acrylic acid)-modified polyurethane film. The amount of encapsulated epidermal growth factor is controlled by adjusting the number of chitosan/epidermal growth factor bilayers. A controlled release of epidermal growth factor from the surface of polyurethane film for a period of five days is achieved with well-retained bioactivity (over 90%) as evidenced by a cell proliferation assay. In an in vitro cellular wounding assay, the cell gap covered with the epidermal growth factor-loaded polyurethane film closes at a rate more than twice as fast as that covered with a control polyurethane film. Fluorescent staining of F-actin reveals that the released epidermal growth factor induces differences in cytoskeletal organization, suggesting that stimulated cell migration also contributes to the close of the cell gap. en_US
dc.description.sponsorship the Manitoba Health Research Council (MHRC) Operating grant the Collaborative Health Research Projects (CHRP) operating grant (Grant no. CHRP 413713-2012) the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery grant (Grant no.: RGPIN/372048-2009) en_US
dc.language.iso en en_US
dc.publisher SAGE Publishing en_US
dc.subject Delivery of epidermal growth factor en_US
dc.subject Layer-by-Layer assembly en_US
dc.subject Wound healing en_US
dc.subject Polyurethane film en_US
dc.title Layer-by-layer assembly of epidermal growth factors on polyurethane films for wound closure en_US
dc.type Article en_US
dc.identifier.doi 10.1177/0885328214523058


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