Layer-by-layer assembly of epidermal growth factors on polyurethane films for wound closure

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dc.contributor.authorKulkarni, Abhilash
dc.contributor.authorDiehl-Jones, William
dc.contributor.authorGhanbar, Sadegh
dc.contributor.authorLiu, Song
dc.date.accessioned2018-10-09T14:45:45Z
dc.date.available2018-10-09T14:45:45Z
dc.date.issued2014-02-13
dc.date.submitted2018-10-08T00:40:33Zen
dc.description.abstractTo facilitate the healing of chronic wounds, growth factors such as epidermal growth factor need to be safely encapsulated for their sustained and effective delivery to the wound bed. Using a layer-by-layer assembly technique, epidermal growth factor is successfully encapsulated on the surface of poly(acrylic acid)-modified polyurethane film. The amount of encapsulated epidermal growth factor is controlled by adjusting the number of chitosan/epidermal growth factor bilayers. A controlled release of epidermal growth factor from the surface of polyurethane film for a period of five days is achieved with well-retained bioactivity (over 90%) as evidenced by a cell proliferation assay. In an in vitro cellular wounding assay, the cell gap covered with the epidermal growth factor-loaded polyurethane film closes at a rate more than twice as fast as that covered with a control polyurethane film. Fluorescent staining of F-actin reveals that the released epidermal growth factor induces differences in cytoskeletal organization, suggesting that stimulated cell migration also contributes to the close of the cell gap.en_US
dc.description.sponsorshipthe Manitoba Health Research Council (MHRC) Operating grant the Collaborative Health Research Projects (CHRP) operating grant (Grant no. CHRP 413713-2012) the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery grant (Grant no.: RGPIN/372048-2009)en_US
dc.identifier.citationKulkarni, A.; Diehl-Jones, W.; Liu, S.* Layer-by-Layer assembly of epidermal growth factors on polyurethane films for wound closure. Journal of Biomaterials Applications, 2014; 29(2): 278-290.en_US
dc.identifier.doi10.1177/0885328214523058
dc.identifier.urihttp://hdl.handle.net/1993/33528
dc.language.isoengen_US
dc.publisherSAGE Publishingen_US
dc.rightsopen accessen_US
dc.subjectDelivery of epidermal growth factoren_US
dc.subjectLayer-by-Layer assemblyen_US
dc.subjectWound healingen_US
dc.subjectPolyurethane filmen_US
dc.titleLayer-by-layer assembly of epidermal growth factors on polyurethane films for wound closureen_US
dc.typeArticleen_US
local.author.affiliationFaculty of Agricultural and Food Sciencesen_US
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