Canola (Brassica napus L.) is economically significant to the Canadian agricultural industry, due to the oil content of the seed though protein quantity and quality have suffered as a result of past breeding efforts to increase oil content. Through quantitative trait loci (QTL) mapping, regions on A3 were discovered controlling 4.6 – 7.2 % of seed oil content and 11.6 – 12.3 % of the phenotypic variation in seed protein content. Two major QTL were discovered on C1 and C2 explaining 12.1 and 15.0 % of the phenotypic variation in cysteine content. Regions on C5 were found controlling arginine, glutamine, isoleucine, leucine and lysine contents explaining 11.0 – 14.0 % of observed variation. The same region also had control over asparagine, phenylalanine, serine and valine content explaining 7.0 – 10.0 % of phenotypic variation. These regions provide useful information for future efforts to enhance seed and meal quality in B. napus.