Role of RAE1 in Arabidopsis thaliana heat tolerance
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Date
2018
Authors
Alharthy, Azizah
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Abstract
Plants need to adapt to environmental conditions such as heat stress. Studies in Arabidopsis thaliana have implicated Damaged DNA Binding protein 1 (DDB1) in plant heat tolerance. DDB1 interacts with DDB1-binding WD40 (DWD) proteins, and six heat-induced DWD genes were identified as candidate genes. Analysis of mutant alleles of these candidates revealed that an RNA EXPORT 1 (rae1) mutant exhibited heat sensitivity. We examined the effect of this allele on the RAE1 transcript and found that RAE1 was still expressed in control conditions, however heat stress resulted in failure of intron 1 splicing in the mutant. In stronger rae1 alleles we were unable to identify homozygotes due to female gametophytic lethality. Nonetheless, heterozygotes of one of these stronger alleles also exhibit increased heat sensitivity. In addition, RAE1 overexpression resulted in heat sensitivity. Thus, both loss and gain of function alleles of RAE1 result in decreased heat tolerance in Arabidopsis.
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