Role of RAE1 in Arabidopsis thaliana heat tolerance

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dc.contributor.authorAlharthy, Azizah
dc.contributor.examiningcommitteeStout, Jake (Biological Sciences) Duncan, Robert (Plant Science)en_US
dc.contributor.supervisorSchroeder, Dana (Biological Sciences)en_US
dc.date.accessioned2018-04-11T20:26:31Z
dc.date.available2018-04-11T20:26:31Z
dc.date.issued2018
dc.date.submitted2018-03-27T18:54:03Zen
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractPlants need to adapt to environmental conditions such as heat stress. Studies in Arabidopsis thaliana have implicated Damaged DNA Binding protein 1 (DDB1) in plant heat tolerance. DDB1 interacts with DDB1-binding WD40 (DWD) proteins, and six heat-induced DWD genes were identified as candidate genes. Analysis of mutant alleles of these candidates revealed that an RNA EXPORT 1 (rae1) mutant exhibited heat sensitivity. We examined the effect of this allele on the RAE1 transcript and found that RAE1 was still expressed in control conditions, however heat stress resulted in failure of intron 1 splicing in the mutant. In stronger rae1 alleles we were unable to identify homozygotes due to female gametophytic lethality. Nonetheless, heterozygotes of one of these stronger alleles also exhibit increased heat sensitivity. In addition, RAE1 overexpression resulted in heat sensitivity. Thus, both loss and gain of function alleles of RAE1 result in decreased heat tolerance in Arabidopsis.en_US
dc.description.noteMay 2018en_US
dc.identifier.urihttp://hdl.handle.net/1993/32955
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectRoleen_US
dc.titleRole of RAE1 in Arabidopsis thaliana heat toleranceen_US
dc.typemaster thesisen_US
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