Show simple item record Sun, Guangping en_US 2007-06-01T19:23:26Z 2007-06-01T19:23:26Z 1999-09-01T00:00:00Z en_US
dc.description.abstract Fibroblast growth factor-2 (FGF-2) is a multifunctional factor which is found in 21-25 kDa high molecular weight (HMW), or 16-18 kDa low molecular weight (LMW) forms, resulting from translation initiation from unconventional CUG- or conventional AUG- start sites, respectively. Previous studies have demonstrated that rat HMW- but not LMW-FGF-2, introduced into cardiac myocytes by gene transfer, caused a distinct nuclear phenotype, characterized by condensed chromatin and formation of several DNA 'clumps' inside the nucleus. In the present study we investigated first whether human HMW FGF-2, sharing 82% homology with its rat counterpart at the CUG-initiated extension, was also capable of eliciting the same phenotype as rat HMW FGF-2. Secondly, we investigated whether the mechanism of chromatin condensation and separation caused by HMW FGF-2 engaged an apoptosis-like, mechanism. Finally, we examined whether the HMW FGF-2 induced nuclear phenotype resulted from a mitosis-like mechanism. (Abstract shortened by UMI.) en_US
dc.format.extent 4375344 bytes
dc.format.extent 184 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language en en_US
dc.language.iso en_US
dc.rights info:eu-repo/semantics/openAccess
dc.title Chromatin condensation and fragmentation caused by CUG-initiated FGF-2 in cardiomyocytes en_US
dc.type info:eu-repo/semantics/masterThesis Human Anatomy & Cell Science en_US Master of Science (M.Sc.) en_US

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