Chromatin condensation and fragmentation caused by CUG-initiated FGF-2 in cardiomyocytes
| dc.contributor.author | Sun, Guangping | en_US |
| dc.date.accessioned | 2007-06-01T19:23:26Z | |
| dc.date.available | 2007-06-01T19:23:26Z | |
| dc.date.issued | 1999-09-01T00:00:00Z | en_US |
| dc.degree.discipline | Human Anatomy and Cell Science | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Fibroblast growth factor-2 (FGF-2) is a multifunctional factor which is found in 21-25 kDa high molecular weight (HMW), or 16-18 kDa low molecular weight (LMW) forms, resulting from translation initiation from unconventional CUG- or conventional AUG- start sites, respectively. Previous studies have demonstrated that rat HMW- but not LMW-FGF-2, introduced into cardiac myocytes by gene transfer, caused a distinct nuclear phenotype, characterized by condensed chromatin and formation of several DNA 'clumps' inside the nucleus. In the present study we investigated first whether human HMW FGF-2, sharing 82% homology with its rat counterpart at the CUG-initiated extension, was also capable of eliciting the same phenotype as rat HMW FGF-2. Secondly, we investigated whether the mechanism of chromatin condensation and separation caused by HMW FGF-2 engaged an apoptosis-like, mechanism. Finally, we examined whether the HMW FGF-2 induced nuclear phenotype resulted from a mitosis-like mechanism. (Abstract shortened by UMI.) | en_US |
| dc.format.extent | 4375344 bytes | |
| dc.format.extent | 184 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.format.mimetype | text/plain | |
| dc.identifier.uri | http://hdl.handle.net/1993/2388 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.title | Chromatin condensation and fragmentation caused by CUG-initiated FGF-2 in cardiomyocytes | en_US |
| dc.type | master thesis | en_US |