Quantitative cell migration analysis of CCR7-mediated lymphocytes migration using a microfluidic device
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Date
2013
Authors
Wu, Xun
Journal Title
Journal ISSN
Volume Title
Publisher
The Royal Society of Chemistry
Abstract
Lymphocyte migration is crucial for adaptive immunity. CCR7 and its ligands mediate the migration and positioning of T cells in lymph nodes but the underlying mechanism is complex. The research in this thesis investigated CCR7-mediated T cell migration using a microfluidics-based approach. A microfluidic method suitable for quantitative migration analysis of genetically modified lymphocyte transfectants was developed. Using this method, I demonstrated chemotaxis of Jurkat transfectants expressing wild-type or C-terminal mutated CCR7 to a CCL19 gradient, and characterized the difference in transfectant migration mediated by wild-type and mutant CCR7. The fluorescent tag allows identification of CCR7-expressing transfectants in cell migration analysis, and microscopy assessment of CCR7 dynamics in migrating cells. Furthermore, my results also showed interesting migratory behaviours of CCR7 Jurkat transfectants in a specific co-existing CCL19 and CCL21 fields. This developed method will be broadly useful for studying cell migration signalling.
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Keywords
microfluidic device, cell migration, chemotaxis, quantitative analysis, lymphocyte, CCR7
Citation
Wu, J., X. Wu and F. Lin (2013). "Recent developments in microfluidics-based chemotaxis studies." Lab Chip 13(13): 2484-2499.