Quantitative cell migration analysis of CCR7-mediated lymphocytes migration using a microfluidic device
| dc.contributor.author | Wu, Xun | |
| dc.contributor.examiningcommittee | Kung, Sam (Immunology) Kormish, Jay (Biological Science) | en_US |
| dc.contributor.supervisor | Lin, Francis (Immunology) Marshall, Aaron (Immunology) | en_US |
| dc.date.accessioned | 2014-08-26T18:20:19Z | |
| dc.date.available | 2014-08-26T18:20:19Z | |
| dc.date.issued | 2013 | en_US |
| dc.degree.discipline | Immunology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Lymphocyte migration is crucial for adaptive immunity. CCR7 and its ligands mediate the migration and positioning of T cells in lymph nodes but the underlying mechanism is complex. The research in this thesis investigated CCR7-mediated T cell migration using a microfluidics-based approach. A microfluidic method suitable for quantitative migration analysis of genetically modified lymphocyte transfectants was developed. Using this method, I demonstrated chemotaxis of Jurkat transfectants expressing wild-type or C-terminal mutated CCR7 to a CCL19 gradient, and characterized the difference in transfectant migration mediated by wild-type and mutant CCR7. The fluorescent tag allows identification of CCR7-expressing transfectants in cell migration analysis, and microscopy assessment of CCR7 dynamics in migrating cells. Furthermore, my results also showed interesting migratory behaviours of CCR7 Jurkat transfectants in a specific co-existing CCL19 and CCL21 fields. This developed method will be broadly useful for studying cell migration signalling. | en_US |
| dc.description.note | October 2014 | en_US |
| dc.identifier.citation | Wu, J., X. Wu and F. Lin (2013). "Recent developments in microfluidics-based chemotaxis studies." Lab Chip 13(13): 2484-2499. | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/23886 | |
| dc.language.iso | eng | en_US |
| dc.publisher | The Royal Society of Chemistry | en_US |
| dc.rights | open access | en_US |
| dc.subject | microfluidic device | en_US |
| dc.subject | cell migration | en_US |
| dc.subject | chemotaxis | en_US |
| dc.subject | quantitative analysis | en_US |
| dc.subject | lymphocyte | en_US |
| dc.subject | CCR7 | en_US |
| dc.title | Quantitative cell migration analysis of CCR7-mediated lymphocytes migration using a microfluidic device | en_US |
| dc.type | master thesis | en_US |