Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens)

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dc.contributor.authorVan Walleghem, Elissa
dc.contributor.examiningcommitteeDocker, Margaret (Biological Sciences) Butler, Micheal (Microbiology)en_US
dc.contributor.supervisorClouthier, Sharon (Biological Sciences) Anderson, Gary (Biological Sciences)en_US
dc.date.accessioned2015-09-11T15:45:30Z
dc.date.available2015-09-11T15:45:30Z
dc.date.issued2013en_US
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractNamao virus (NV) was associated with mortality in lake sturgeon Acipenser fulvescens reared as part of a conservation stocking program for this endangered species in Manitoba, Canada. The virus itself was large, doubly encapsidated and icosahedral-shaped. Phylogenetic analyses using the major capsid protein showed that NV and other epitheliotropic sturgeon nucleo-cytoplasmic large DNA viruses shared a common evolutionary past and formed a distinct evolutionary lineage within Megavirales. Three PCR tests were developed and their analytical performance was validated for detection of these viruses. Testing of wild sturgeon revealed that NV is endemic in the Nelson River water basin in Manitoba. Bath exposure resulted in transmission of NV to healthy sturgeon. The gills appeared to be the initial site of infection with virus persisting in the head skin tissue for up to 62 days. The molecular tests will be useful tools for disease management in sturgeon conservation stocking programs.en_US
dc.description.noteOctober 2015en_US
dc.identifier.citationClouthier SC, VanWalleghem E, Copeland S, Klassen C, Hobbs G, Nielsen O, Anderson ED (2013) Namao virus, a new unclassified species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens. Dis Aquat Org 102:195-209en_US
dc.identifier.urihttp://hdl.handle.net/1993/30754
dc.language.isoengen_US
dc.publisherDiseases of Aquatic Organismsen_US
dc.rightsopen accessen_US
dc.subjectNamao virusen_US
dc.subjectNCLDVen_US
dc.subjectLake Sturgeonen_US
dc.subjectEndangered speciesen_US
dc.subjectqPCRen_US
dc.subjectDiagnostic PCRen_US
dc.subjectReal-time PCRen_US
dc.subjectMimiviridaeen_US
dc.titleAnalytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens)en_US
dc.typemaster thesisen_US
local.subject.manitobayesen_US
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