A comparative study of the antioxidant potential and metabolic profiling of lingonberry (Vaccinium vitis idaea) from Northern Manitoba and Newfoundland
| dc.contributor.author | Bhusal, Aakangchhya | |
| dc.contributor.examiningcommittee | Siow Chris, (Physiology and Pathophysiology) Aliani Michel, (Human Nutritional Sciences) | en_US |
| dc.contributor.supervisor | Brown Dan,(Human Nutritional Sciences) | en_US |
| dc.date.accessioned | 2016-04-13T15:49:23Z | |
| dc.date.available | 2016-04-13T15:49:23Z | |
| dc.date.issued | 2016 | |
| dc.degree.discipline | Human Nutritional Sciences | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Lingonberries (Vaccinium vitis-idaea) are edible fruit that belong to the Ericaceae plant family. A growing body of evidence suggests that consumption of berries provide considerable health benefits due to their high polyphenols, antioxidants, vitamins and mineral content. Therefore, it is beneficial to evaluate the antioxidant capacity of berries in order to select lines with higher antioxidant capacity. The aim of this study was to determine the antioxidant capacity, total phenolics, total anthocyanins, anti-apoptotic effect and the metabolomic profile of lingonberries from Northern Manitoba (wild) and Newfoundland (greenhouse-grown). A total of 159 lingonberry samples were collected from Northern Manitoba (Lynn Lake and Flin Flon) and Newfoundland. Samples were freeze dried, lyophilized, ground into powder and extracted with solvent methanol. Total anthocyanins were determined by the pH differential method. Total phenolics were evaluated by the Folin-Ciocalteau’s assay, the anti-apoptotic effect was measured fluorescently and the oxygen radical absorbance capacity (ORAC) assay was utilized to evaluate the antioxidant capacity. Our study showed that Northern Manitoba-grown lingonberries contain a higher level of anthocyanins and phenolics compared to Newfoundland greenhouse-grown lingonberries. Results show that Northern Manitoba- grown lingonberries have higher antioxidant capacity compared to ones grown in Newfoundland. Findings from the study suggest that berries grown in green house conditions and extreme climatic conditions have different antioxidant capacity, anti-apoptotic effects, total phenolics and total anthocyanins; that is, more extreme climates may result in berries with higher antioxidant capacity, total phenolics and anthocyanins. | en_US |
| dc.description.note | May 2016 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/31214 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | lingonberry | en_US |
| dc.subject | antioxidant | en_US |
| dc.subject | comparative study | en_US |
| dc.title | A comparative study of the antioxidant potential and metabolic profiling of lingonberry (Vaccinium vitis idaea) from Northern Manitoba and Newfoundland | en_US |
| dc.type | master thesis | en_US |
| local.subject.manitoba | yes | en_US |