This study aimed to evaluate the chemical composition and digestibility of expeller soybean meal (SBM) derived from Manitoba-grown soybean as a potential protein source for broiler chickens. The utilization of locally produced Manitoba SBM could offer a cost-effective and sustainable alternative to imported soybean meal. The standardized ileal amino acid digestibility (SIAAD) of Manitoba SBM was assessed through a digestibility study using a nitrogen-free diet (NFD). Three samples of Manitoba SBM and one sample of SBM from Ontario-grown seeds were analyzed and incorporated into diets as the sole source of nitrogen. Day-old Ross 308 chickens (n=320) were allocated to 64 cages with 5 birds per cage and fed a starter diet for 14 days. On day 15, the birds were randomly assigned to one of the five treatments, with 10 replicate cages per treatment, and stayed on trial for 5 days. The crude protein (CP) concentration of the Manitoba SBM samples ranged from 40.4-41.5% and the Ontario sample had a CP concentration of 47.9% on dry matter basis. The fat content of Manitoba and Ontario samples ranged from 10.6-11.3% and 9%, respectively. The total non-starch polysaccharides in the Manitoba and Ontario samples were 17.4-18% and 15.5%, respectively. Raffinose-family oligosaccharides were present at levels of 6.5- 8.7% in the Manitoba SBM samples and 6.2% in Ontario sample. The SIAAD of Manitoba SBM was similar to the control, except for histidine, methionine, and cysteine (P < 0.05) which were lower in two Manitoba samples. The mean standardized ileal CP digestibility of Manitoba SBM was 78.1%, compared to 81.1% for Ontario samples, and the difference was not statistically significant. The overall SIAAD values of Manitoba SBM were found to be lower than those reported in the literature for expeller-pressed SBM, explained by increased antinutritional factor content and potential differences in processing techniques. In conclusion, Manitoba SBM holds promise as a viable protein ingredient for poultry nutrition, however further studies are necessary to understand its full potential.