The relationships between DNA methylation levels and chorionicity in newborn twins

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dc.contributor.authorTran, Terry
dc.contributor.examiningcommitteeRastegar, Mojgan (Biochemistry and Medical Genetics) Acar, Elif (Statistics)en_US
dc.contributor.supervisorLiu, Michelle XiaoQing (Biochemistry and Medical Genetics) Simard, Louise (Biochemistry and Medical Genetics)en_US
dc.date.accessioned2015-04-07T16:59:27Z
dc.date.available2015-04-07T16:59:27Z
dc.date.issued2015-04-07
dc.degree.disciplineBiochemistry and Medical Geneticsen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractMonozygotic (MZ) twins are currently used in epigenetic studies as one homogenous group. However, there are two MZ twin types: dichorionic (DC) and monochorionic (MC). We hypothesize that DCMZ twins are more similar epigenetically, compared to MCMZ twins, due to earlier zygote splitting, a higher degree of birth outcome similarity, or both. We recruited 220 newborn twins and obtained genome-wide DNA methylation profiles for 48 twins. Intraclass correlation coefficients (ICC) and linear mixed models were used to investigate the relationships between DNA methylation levels and chorionicity. DCMZ twins tended to have longer gestational age, larger birth weight, and smaller birth length discordance. DCMZ twins had more similar DNA methylation profiles than MCMZ twins (ICC=0.21 vs. 0.13), after adjusting for birth outcomes. Additionally, we identified 5,170 CpG sites with different DNA methylation levels between DCMZ and MCMZ twins. This study highlights the importance of incorporating chorionicity information in epigenetic twin studies.en_US
dc.description.noteMay 2015en_US
dc.identifier.urihttp://hdl.handle.net/1993/30353
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectDNA Methylationen_US
dc.subjectTwinsen_US
dc.subjectChorionicityen_US
dc.subjectEpigeneticsen_US
dc.subjectHumanMethylation450en_US
dc.subjectBirth Weighten_US
dc.titleThe relationships between DNA methylation levels and chorionicity in newborn twinsen_US
dc.typemaster thesisen_US
local.subject.manitobayesen_US
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