Structural characterization of phenolic compounds in canola meal: impact of high pressure and temperature

dc.contributor.authorNandasiri, Hewa Madihe Annakkage Ruchira
dc.contributor.examiningcommitteeAluko, Rotimi (Food and Human Nutritional Sciences)en_US
dc.contributor.examiningcommitteeNetticadan, Thomas (Physiology and Pathophysiology)en_US
dc.contributor.examiningcommitteeShahidi, Fereidoon (Memorial University)en_US
dc.contributor.supervisorEskin, N. A Michael (Food and Human Nutritional Sciences) Eck, Peter (Food and Human Nutritional Sciences)en_US
dc.date.accessioned2021-09-17T22:02:09Z
dc.date.available2021-09-17T22:02:09Z
dc.date.copyright2021-09-17
dc.date.issued2021-07en_US
dc.date.submitted2021-09-02T19:08:55Zen_US
dc.date.submitted2021-09-17T21:19:14Zen_US
dc.degree.disciplineFood and Human Nutritional Sciencesen_US
dc.degree.levelDoctor of Philosophy (Ph.D.)en_US
dc.description.abstractCanola is the major oilseed crop of Canada. After extraction of the oil, a substantial amount of meal is generated. To date, however, the meal has received little attention from both industry and researchers. Despite its nutrition and functional potential, utilization of canola meal has been limited by its high fiber content and antinutritional components. Nevertheless, it has a rich phenolic profile, that could be a valuable source of nutraceuticals. The presence of large amounts of sinapine and sinapic acid in canola meal and its conversion to canolol has received a lot of attention because of its powerful antioxidant and anticancer properties. What is desired, however, is an effective method for their extraction. The use of pressurized (1,500 psi) high-temperature processing is ideal for the extraction of phenolic compounds from such by-products including the meal. This study investigated the application of accelerated solvent extraction (ASE) as a method for extracting phenolic compounds from canola meal. Different extraction temperatures, solvents, and solvent concentrations were used to establish the optimum extraction conditions for major sinapates and other phenolic compounds. Changes in total phenolics, total flavonoids, and antioxidant capacity were quantified as a measure of extraction efficiency. The ASE method is an efficient method for extracting phenolic compounds from the meal. In addition to ASE, RapidOxy®100, an automated commercial instrument, was adapted as a solvent-free pre-treatment of canola meal prior to the ultrasonic extraction of phenolic compounds. It also proved to be a novel and versatile method for enhancing the extraction of phenolic compounds. Both techniques have the potential for the mainstream production of phenolic-rich extracts as a valuable source of natural bioactive compounds and nutraceuticals from underutilized agricultural by‐products such as canola meal.en_US
dc.description.noteOctober 2021en_US
dc.identifier.urihttp://hdl.handle.net/1993/36003
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectCanola mealen_US
dc.subjectHigh temperatureen_US
dc.subjectHigh pressureen_US
dc.subjectCanololen_US
dc.subjectSinapineen_US
dc.subjectSinapic aciden_US
dc.subjectKaempferolen_US
dc.titleStructural characterization of phenolic compounds in canola meal: impact of high pressure and temperatureen_US
dc.typedoctoral thesisen_US
local.subject.manitobayesen_US
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