Properties of layer-by-layer single-cell encapsulation and the potential applications in transplantation therapy for neurologic disorders

Li, Wenyan

Properties of layer-by-layer single-cell encapsulation and the potential applications in transplantation therapy for neurologic disorders

dc.contributor.author	Li, Wenyan
dc.contributor.examiningcommittee	Xing, Malcolm (Mechanical Engineering) Marzban, Hassan (Human Anatomy and Cell Science) Gong, Yuewen (Pharmacy) Song, Weihong (University of British Columbia)	en_US
dc.contributor.supervisor	Kong, Jiming (Human Anatomy and Cell Science)	en_US
dc.date.accessioned	2019-01-09T21:18:19Z
dc.date.available	2019-01-09T21:18:19Z
dc.date.issued	2018-12-05	en_US
dc.date.submitted	2018-12-05T17:03:25Z	en
dc.degree.discipline	Human Anatomy and Cell Science	en_US
dc.degree.level	Doctor of Philosophy (Ph.D.)	en_US
dc.description.abstract	Layer-by-layer (LbL) self-assembly is a rapidly developing biomaterial technique for fabricating multilayered structure on substrates. In this project, we report the encapsulation of single neural stem cells (NSCs) using the LbL self-assembly technique with gelatin and alginate. The encapsulation did not affect normal functions of the encapsulated NSCs. When insulin-like growth factor-1 was loaded on materials, its release significantly enhanced the proliferation of the encapsulated NSCs. Permeability of the LbL encapsulation was investigated on PC12 cells encapsulated with gelatin and hyaluronic acid (HA). The permeability was inversely proportional to molecular weight of FITC-dextran (FD), material concentration, and number of layers. Encapsulation with 0.5% of the materials for 8 layers was proven to effectively inhibit the cell apoptosis induced by tumor necrosis factor-α (TNF-α). Since the encapsulation structure creates a favorable microenvironment for cells to survive and function, it holds significant potential for cell therapies for neurologic disorders.	en_US
dc.description.note	February 2019	en_US
dc.identifier.citation	Wenyan Li, Guohui Zhang, Teng Guan, Xiaosha Zhang, Ali Khosrozadeh, Malcolm Xing* and Jiming Kong*. Manipulable Permeability of Nanogel Encapsulation on Cells Exerts Protective Effect against TNF-α-Induced Apoptosis. ACS Biomater. Sci. Eng. 2018, 4, 2825−2835.	en_US
dc.identifier.citation	Wenyan Li , Teng Guan , Xiaosha Zhang , Ziyuan Wang , Meng Wang , Wen Zhong , Hua Feng , Malcolm M.Q. Xing* , and Jiming Kong*. The effect of layer-by-layer assembly coating on the proliferation and differentiation of neural stem cells. ACS Applied Materials & Interfaces, 2015, 7(5), 3018-3029.	en_US
dc.identifier.citation	Shilun Zuo, Wenyan Li, Qiang Li, Hengli Zhao, Jun Tang, Qianwei Chen, Xin Liu, John H. Zhang, Yujie Chen* and Hua Feng*. Protective effects of Ephedra sinica extract on blood– brain barrier integrity and neurological function correlate with complement C3 reduction after subarachnoid hemorrhage in rats. Neuroscience Letters, 2015, 609, 216-222.	en_US
dc.identifier.citation	Shuhan Liu, Alex C.-T. Ko, Wenyan Li, Wen Zhong* and Malcolm Xing*. NIR initiated and pH sensitive single-wall carbon nanotubes for doxorubicin intracellular delivery. Journal of Materials Chemistry B, 2014, 2, 1125-1135.	en_US
dc.identifier.citation	Chengren Li, Teng Guan, Xueping Chen, Wenyan Li, Qiyan Cai, Jianqin Niu, Lan Xiao* and Jiming Kong*. BNIP3 mediates pre-myelinating oligodendrocyte cell death in hypoxia and ischemia. Journal of Neurochemistry, 2013, 127, 426-433.	en_US
dc.identifier.uri	http://hdl.handle.net/1993/33684
dc.language.iso	eng	en_US
dc.rights	open access	en_US
dc.subject	Layer-by-layer	en_US
dc.subject	Cell encapsulation	en_US
dc.subject	Drug delivery	en_US
dc.subject	Permeability	en_US
dc.subject	Microenvironment regulation	en_US
dc.title	Properties of layer-by-layer single-cell encapsulation and the potential applications in transplantation therapy for neurologic disorders	en_US
dc.type	doctoral thesis	en_US
local.subject.manitoba	yes	en_US