Quantitative Trait Loci Controlling Sclerotinia Stem Rot Resistance and Seed Glucosinolate Content of Oilseed Rape (Brassica napus L.)

Simple item page
dc.contributor.authorLiu, Jun
dc.contributor.examiningcommitteeMcVetty, Peter (Plant Science) Belmonte, Mark (Biological Sciences) Wu, Chunren (Plant Science) Li, Genyi (Plant Science) Weselake, Randall (University of Alberta)en_US
dc.contributor.supervisorLi, Genyi (Plant Science)en_US
dc.date.accessioned2016-09-09T17:36:15Z
dc.date.available2016-09-09T17:36:15Z
dc.date.issued2016en_US
dc.degree.disciplinePlant Scienceen_US
dc.degree.levelDoctor of Philosophy (Ph.D.)en_US
dc.description.abstractCanola/rapeseed (Brassica napus L.) is a major oilseed crop worldwide. However, its production is largely affected by the fungal disease Sclerotinia stem rot as well as seed glucosinolates. So far the genetic mechanisms controlling these two traits have been poorly understood. In the present study, three bi-parental doubled haploid B. napus populations M730, M692 and ZT were grown in either natural or artificial environments and genotyped using the Brassica 60K InfiniumĀ® SNPs and/or sequence related amplified polymorphisms. Three genetic linkage maps covered 2,597.7 cM, 2,474.1 cM and 1,731.6 cM in 19 chromosomes for M730, M692 and ZT, respectively. Plants were inoculated with Sclerotinia sclerotiorum mycelia on stems at the reproductive stage to evaluate their resistivity. Four aliphatic glucosinolates and one indolic glucosinolate were detected in the seeds using high-performance liquid chromatography. 4-hydroxy-3-indolylmethyl predominated over aliphatic glucosinolates in canola, but inversely constituted a small portion of total glucosinolate content in semi-winter rapeseed. In rapeseed, 2-hydroxy-3-butenyl predominated in 4C aliphatic glucosinolates, which in turn predominated in total aliphatic glucosinolates, which likewise predominated in total glucosinolate content. QTLs regulating major glucosinolates were located on chromosome A9 for high glucosinolate content populations M730 and ZT, and on chromosome C7 for low glucosinolate content population M692. Major QTLs for Sclerotinia stem rot resistance were located on chromosomes A7 and C6 in M730, on chromosomes A3 and A7 in ZT, while no major QTLs were found in M692. Additive genetic effect was the major factor explaining phenotypic variations of the two traits. No direct genetic relationship was observed between Sclerotinia stem rot resistance in adult plants and seed glucosinolates in B. napus. The findings in the studies could be used to formulate breeding and research strategies in B. napus and the major QTLs controlling the two traits and their closely linked SNP markers could be validated over wide germplasm and used in marker assisted selection.en_US
dc.description.noteOctober 2016en_US
dc.identifier.urihttp://hdl.handle.net/1993/31662
dc.language.isoengen_US
dc.publisherAustralian Journal of Plant Scienceen_US
dc.rightsopen accessen_US
dc.subjectBrassica napusen_US
dc.subjectSclerotinia stem roten_US
dc.subjectglucosinolateen_US
dc.subjectQuantitative trait locien_US
dc.subjectSclerotinia sclerotiorumen_US
dc.titleQuantitative Trait Loci Controlling Sclerotinia Stem Rot Resistance and Seed Glucosinolate Content of Oilseed Rape (Brassica napus L.)en_US
dc.typedoctoral thesisen_US
local.subject.manitobayesen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
jun_liu.pdf
Size:
5.52 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.2 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
FGS - Electronic Theses and Practica
Manitoba Heritage Theses