The study of cellular behavior necessitates precise and efficient tools for measuring various cellular properties. This thesis focuses on the development and implementation of a novel platform for simultaneous dielectric and optical properties characterization of single cells and particles. The motivation behind this work stems from the need for accurate and efficient tools to study cellular behavior and recent advancements in sensors and electronics. Dielectric properties of cells and particles have significant value in various applications, including cell identification and sorting, disease diagnosis and monitoring, drug discovery and development, as well as provide valuable insights into their structural and functional characteristics. The objectives of this thesis project were to enhance the optical transit differential dielectrophoresis cytometer strategy, enable rapid deployment of automated experiments, and provide a reliable platform for future data collection. These objectives were achieved through the development of two generations of hardware platforms. The first generation hardware platform laid the foundation for further improvements and presented the potential of the method. Building upon this, the second generation hardware platform addressed the limitations identified in the first generation system, resulting in a more reliable and flexible system with enhanced specifications and performance. This platform enables faster and more efficient data collection, provides higher resolution particle size information, allows for multiwavelength captures, and enables simultaneous measurement of multiple DEP frequencies. The experimental and simulated results presented in this thesis demonstrate the system's capabilities, performance, and potential for further development. The platforms demonstrated the ability to differentiate particles based on particle size, shown promise in estimating cell viability and differentiating between different types of cancer cells based on their dielectric properties. These achievements emphasize the system's relevance in cellular research, diagnostics, and cancer studies. The findings of this thesis highlights the potential and versatility of the optical transit parallel single-cell differential DEP cytometer strategy. The developed hardware platforms provide a solid foundation for future research and development in this field. This work contributes to advancing cellular research, diagnostics, and cancer studies, providing valuable insights into cellular behavior and opening new avenues for further exploration.