Nondestructive molecular sex determination of free-ranging star-nosed moles (Condylura cristata)

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dc.contributor.authorPrice, Nadine
dc.contributor.examiningcommitteeRoth, James (Biological Sciences) Koper, Nicola (Natural Resources Institute)en_US
dc.contributor.supervisorCampbell, Kevin (Biological Sciences) Willis, Craig (Biological Sciences)en_US
dc.date.accessioned2014-01-15T21:13:23Z
dc.date.available2014-01-15T21:13:23Z
dc.date.issued2014-01-15
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractMolecular techniques, particularly noninvasive genetic sampling (NGS) and nondestructive sampling (NDS), are increasingly being used as tools to study the ecology of free-ranging mammals. A specific application of these methods is the molecular sexing of species for which external sex differentiation is challenging. Star-nosed moles (Condylura cristata) are a little-studied species in which females possess a peniform clitoris making them externally indistinguishable from males. To my knowledge, no studies have employed NDS to study any aspect of their ecology. I therefore sequenced fragments of one X-chromosome (Zfx) and two Y-chromosome (Sry and Zfy) genes from known-sex specimens, and designed species-specific primers to co-amplify these loci from hair, claw and fecal samples of 16 star-nosed moles. I found all tissue types were highly (90-100%) reliable for sex determination. I envision that this NDS method will facilitate future capture-and-release studies on the natural history and social structure of this fascinating, semi-aquatic mammal.en_US
dc.description.noteFebruary 2014en_US
dc.identifier.urihttp://hdl.handle.net/1993/23251
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectstar-nosed moleen_US
dc.subjectCondylura cristataen_US
dc.subjectnondestructiveen_US
dc.subjectnoninvasiveen_US
dc.subjectmolecular sex determinationen_US
dc.subjectTalpidaeen_US
dc.subjectSryen_US
dc.subjectZfxen_US
dc.subjectZfyen_US
dc.subjectmolesen_US
dc.titleNondestructive molecular sex determination of free-ranging star-nosed moles (Condylura cristata)en_US
dc.typemaster thesisen_US
local.subject.manitobayesen_US
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