Role of thioredoxin system in regulation of neural stem cell proliferation and differentiation
| dc.contributor.author | Iqbal, Mohamed Ariff | |
| dc.contributor.examiningcommittee | Karimi, Soheila (Physiology and Pathophysiology) Doucette, Christine (Physiology and Pathophysiology) Wang, Jun-Feng (Pharmacology and Therapeutics) | en_US |
| dc.contributor.supervisor | Eftekharpour, Eftekhar (Physiology and Pathophysiology) | en_US |
| dc.date.accessioned | 2016-09-19T17:02:23Z | |
| dc.date.available | 2016-09-19T17:02:23Z | |
| dc.date.issued | 2016 | |
| dc.degree.discipline | Physiology and Pathophysiology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Neural precursor cells (NPCs) have been a hot research topic because of their regenerative potentials. These cells maintain a remarkable capacity for continuous neurogenesis throughout the life and therefore have inspired the quest for a cure towards central nervous system (CNS) repair, however their clinical application is severely restricted due to their limited proliferation. The role of Thioredoxin-1 as a neuroprotective antioxidant protein has been shown previously. This multifaceted protein has also been linked to increase in NPCs proliferation. For my MSc thesis, I hypothesized that enhancing Trx1 levels using a novel intracellular protein delivery approach may enhance cell proliferation and alter differentiation of NPCs. Using immunocytochemistry and Western blotting, I showed that Trx promotes cell proliferation and potentiates oligodendrogenesis in these cells. I have also examined downstream signaling mechanisms involved in these processes. My studies indicate that Trx therapy may represent a viable approach for induction of NPCs proliferation. | en_US |
| dc.description.note | October 2016 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/31813 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Neural stem cells | en_US |
| dc.subject | Oxidative stress | en_US |
| dc.subject | Antioxidants | en_US |
| dc.subject | Thioredoxin system | en_US |
| dc.title | Role of thioredoxin system in regulation of neural stem cell proliferation and differentiation | en_US |
| dc.type | master thesis | en_US |