Monitoring Dielectric Properties of Single MRC5 Cells and Oligomycin Treated Chinese Hamster Ovary Cells Using a Dielectrophoretic Cytometer
| dc.contributor.author | Saboktakin Rizi, Bahareh | |
| dc.contributor.examiningcommittee | Bridges, Gregory (Electrical and Computer Engineering) Xing, Malcolm (Mechanical Engineering) | en_US |
| dc.contributor.supervisor | Thomson, Douglas (Electrical and Computer Engineering) | en_US |
| dc.date.accessioned | 2014-09-17T22:08:38Z | |
| dc.date.available | 2014-09-17T22:08:38Z | |
| dc.date.issued | 2014-09-17 | |
| dc.degree.discipline | Electrical and Computer Engineering | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | We have employed a differential detector combined with dielectrophoretic (DEP) translation in a microfluidic channel to monitor dielectric response of single cells and particularly to track phenomenon related to apoptosis. Two different cell lines were studied; Chinese hamster ovary cells (CHO) and MRC5 cells. Dielectric response was quantified by a factor called Force Index. Force Index was studied statistically to identify apoptotic subpopulations. Another direction of this work was to monitor changes in the cytoplasm conductivity following inhibition of mitochondrial ATP production by Oligomycin. To make the DEP response mostly sensitive to the cytoplasm conductivity, medium conductivity and DEP frequency were adjusted such that Clausius Mossotti factor and hence DEP response become less sensitive to cell radius. Chinese hamster ovary cells were used in this work and the impact of different concentrations of Oligomycin has been studied. We show that following exposure to Oligomycin at 8 μg/ml, cytoplasm conductivity drops. The majority of the changes takes place within one hour of exposure to the drug. Furthermore, double shell models has been used to estimate cytoplasm conductivity in a medium with conductivity of 0.42 S/m and the drop in the cytoplasm conductivity following treatment with Oligomycin was estimated to be ≈ 0.16 S/m. The magnitude of the decrease in the cytoplasm conductivity is evidence that Glycolysis is active as an energy production pathway within the cell. This approach can be used to quantify Glycolysis versus mitochondria ATP production which has an application in Warburg effect in cancer cells and monitoring bioprocesses. | en_US |
| dc.description.note | February 2015 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/24054 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Dielectrophoresis | en_US |
| dc.subject | Single cell analysis | en_US |
| dc.subject | Oligomycin | en_US |
| dc.subject | Cytoplasm conductivity | en_US |
| dc.subject | ATP inhibition | en_US |
| dc.subject | Chinese Hamster Ovary Cells | en_US |
| dc.title | Monitoring Dielectric Properties of Single MRC5 Cells and Oligomycin Treated Chinese Hamster Ovary Cells Using a Dielectrophoretic Cytometer | en_US |
| dc.type | master thesis | en_US |