Environmental DNA monitoring of invasive zebra mussels: method design, monitoring tool comparisons, refinement of methods, and considerations for management

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dc.contributor.authorGingera, Timothy
dc.contributor.examiningcommitteeWhyard, Steve (Biological Sciences) Long, Jeff (Manitoba Water Stewardship Division)en_US
dc.contributor.supervisorDocker, Margaret (Biological Sciences) Reist, James (Biological Sciences)en_US
dc.date.accessioned2018-04-12T16:25:45Z
dc.date.available2018-04-12T16:25:45Z
dc.date.issued2017-03-29en_US
dc.date.submitted2018-03-30T18:55:19Zen
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractInterest in environmental DNA (eDNA) for the detection of aquatic invasive species (AIS) is increasing. Considering the invasion of zebra mussels Dreissena polymorpha into Lake Winnipeg in 2013, the development of eDNA monitoring methods may help managers prevent further spread of this AIS. For this thesis, sensitive and species-specific eDNA quantitative PCR assays and field/laboratory protocols were developed to be used for monitoring in western North America. These eDNA methods were found to be more sensitive for detection than was plankton netting for presence/absence of veligers. Environmental DNA target gene concentration and veliger abundance appear to be positively correlated. Furthermore, veliger abundance may account for much of the variation in eDNA detections. Refinement of eDNA methods is also presented here to improve detection. The work in this thesis provides considerations and guidelines for managers using eDNA as a detection tool for zebra mussels.en_US
dc.description.noteMay 2018en_US
dc.identifier.citationGingera, T.D., Bajno, R., Docker, M.F., Reist, J.D. (2017) Environmental DNA as a detection tool for zebra mussels Dreissena polymorpha (Pallas, 1771) at the forefront of an invasion event in Lake Winnipeg, Manitoba, Canada. Management of Biological Invasions, 8(3), 287-300.en_US
dc.identifier.urihttp://hdl.handle.net/1993/32963
dc.language.isoengen_US
dc.publisherManagement of Biological Invasionsen_US
dc.rightsopen accessen_US
dc.subjectZebra musselsen_US
dc.subjectQuagga musselsen_US
dc.subjectEnvironmental DNAen_US
dc.subjectMonitoringen_US
dc.subjectQuantitative PCRen_US
dc.titleEnvironmental DNA monitoring of invasive zebra mussels: method design, monitoring tool comparisons, refinement of methods, and considerations for managementen_US
dc.typemaster thesisen_US
local.subject.manitobayesen_US
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