Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding
| dc.contributor.author | Renaud, Anais Krystel | |
| dc.contributor.examiningcommittee | Piercey-Normore, Michele (Biological Sciences) Smith, Marjorie (Agriculture and Agri-Food Canada) | en_US |
| dc.contributor.supervisor | Savage, Jade (Bishop's University, Biological Sciences) Galloway, Terry (Entomology) | en_US |
| dc.date.accessioned | 2012-01-05T16:34:01Z | |
| dc.date.available | 2012-01-05T16:34:01Z | |
| dc.date.issued | 2012-01-05 | |
| dc.degree.discipline | Entomology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | A 2007 survey of muscid flies from Churchill, Manitoba, Canada, yielded 155 species. The diversity of this contemporary assemblage was compared to that of an historical (pre-1965) assemblage. Few differences were found between assemblages for material collected by net sweeping and most non-overlapping species between time periods were rare in samples and/or collected by different methods. DNA barcoding was used as a tool to assist with the identification of Muscidae specimens. Performance of the cytochrome c oxidase subunit 1 gene (COI) in the discrimination of muscid species was assessed and correspondence levels of 98.6% were established between species limits recovered by DNA barcode clusters and morphology. I conclude that the great majority of species limits currently accepted in the literature are adequate for Muscidae and that DNA barcoding is a useful identification tool for this family. | en_US |
| dc.description.note | February 2012 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/5029 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Insects | en_US |
| dc.subject | Arctic | en_US |
| dc.title | Biodiversity of the Muscidae (Diptera) from Churchill, Manitoba, Canada, with taxonomic issues revealed or resolved by DNA barcoding | en_US |
| dc.type | master thesis | en_US |
| local.subject.manitoba | yes | en_US |