Drinking water in First Nation communities: occurrence of bacteria and identification of the resistome.
| dc.contributor.author | Murdock, Anita | |
| dc.contributor.examiningcommittee | Brassinga, Karen (Microbiology) | en_US |
| dc.contributor.examiningcommittee | Sparling, Richard (Microbiology) | en_US |
| dc.contributor.supervisor | Kumar, Ayush | |
| dc.contributor.supervisor | Farenhorst, Annemieke | |
| dc.date.accessioned | 2023-06-01T19:55:38Z | |
| dc.date.available | 2023-06-01T19:55:38Z | |
| dc.date.copyright | 2022-07-28 | |
| dc.date.issued | 2022-07-28 | |
| dc.date.submitted | 2022-07-28T15:11:26Z | en_US |
| dc.date.submitted | 2022-07-28T19:14:43Z | en_US |
| dc.degree.discipline | Microbiology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Indigenous populations living on reserves in Canada today are still experiencing uneven access to drinking water services. Past studies of First Nation communities have observed microbiological contamination where no drinking water advisory was issued for fecal indicator bacteria (FIB) and detection of emerging contaminants: antibiotic resistance genes (ARG). The first study sought to observe fluctuations in the distribution system of traditional coliform indicators against antibiotic resistance genes as well as Campylobacter spp. quantification. Results found repeated detection of indicator bacteria from drinking water samples in homes with concrete cisterns in community B, as well as in community D up to 600 CFU/ 100 mL coliforms. Both Campylobacter coli and Campylobacter jejuni, diarrheal infection culprits, were also observed in instances without the previous or same-time detection of coliform, including at sampling sites like treated water from taps at the water treatment plant and piped homes. ARGs were more apparent in post-treated water from community B than from community D and in water treatment plant and piped samples. The second study sought to characterize ARGs through shotgun metagenomics to establish the resistome from water. Results determined pre-treated source water samples were characteristically distinct from post-treated cistern water from each community suggesting the presence of an alternative source of contamination for stored drinking water. Indigenous nations continue to experience water insecurity and these study results reinforce community concerns over water quality thus we aim to support change led within communities for strengthening the management of water distribution to homes. | en_US |
| dc.description.sponsorship | Northern Scientific Training Program | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/37370 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Drinking water | en_US |
| dc.subject | Antibiotic resistance | en_US |
| dc.subject | Resistome | en_US |
| dc.subject | Source water | en_US |
| dc.subject | First Nation | en_US |
| dc.subject | Cistern-held water | en_US |
| dc.title | Drinking water in First Nation communities: occurrence of bacteria and identification of the resistome. | en_US |
| dc.type | master thesis | en_US |
| local.subject.manitoba | yes | en_US |
| oaire.awardNumber | 152945 | en_US |
| oaire.awardTitle | Studying microbial load and antibiotic resistance genes in source and drinking water from First Nation Reserves | en_US |
| oaire.awardURI | https://webapps.cihr-irsc.gc.ca/decisions/p/project_details.html?applId=356359&lang=en | en_US |
| project.funder.identifier | https://doi.org/10.13039/501100000024 | en_US |
| project.funder.name | Canadian Institutes of Health Research | en_US |