Evaluation of co-culture sustainability and hydrogen production in an integrated fermentative microbial electrolysis cell
| dc.contributor.author | Wrana, Nathan | |
| dc.contributor.examiningcommittee | Cicek, Nazim (Biosystems Engineering) Sparling, Richard (Microbiology) | en |
| dc.contributor.supervisor | Levin, David (Biosystems Engineering) | en |
| dc.date.accessioned | 2011-04-07T18:02:51Z | |
| dc.date.available | 2011-04-07T18:02:51Z | |
| dc.date.issued | 2011-04-07T18:02:51Z | |
| dc.degree.discipline | Biosystems Engineering | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | The relationship between the cellulolytic Clostridium termitidis and the electrogenic Geobacter sulfurreducens was evaluated in terms of co-culture sustainability and hydrogen production. Batch co-culture experiments in triplicate balch tubes were conducted using cellobiose as the sole carbon source and fumarate as a terminal electron acceptor. Despite high initial concentrations of acetate, no formate and very low H2 concentrations were detected, supporting the hypothesis that a syntrophic association exists between both bacteria. Co-culture growth characterization experiments were repeated in three microbial electrolysis cells and cellobiose as the sole carbon source. Initially, 9.7 mol-H2 mol-1-glucose was produced. However, a sustainable co-culture could not be maintained despite efforts to reduce reactor temperature and triple the medium’s buffering capacity. Strategies to achieve a sustainable co-culture are to minimize the carbon flux through C. termitidis by using complex substrates, maintain neutral operating conditions, and introduce acetogenic bacteria to control the flux of metabolic intermediates. | en |
| dc.description.note | May 2011 | en |
| dc.format.extent | 39665920 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.citation | Wrana, N., Sparling, R., Cicek, N., and D.B. Levin. (2010). Hydrogen gas production in a microbial electrolysis cell by electrohydrogenesis. Journal of Cleaner Production. 18(1): S105-S111. | en |
| dc.identifier.uri | http://hdl.handle.net/1993/4456 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Hydrogen | en |
| dc.subject | Microbial electrohydrogenesis | en |
| dc.subject | Co-culture | en |
| dc.subject | Fermentation | en |
| dc.title | Evaluation of co-culture sustainability and hydrogen production in an integrated fermentative microbial electrolysis cell | en |
| dc.type | master thesis | en_US |