Manitoba lingonberry (Vaccinium vitis-idaea) protects against ischemia-reperfusion injury

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dc.contributor.authorIsaak, Cara
dc.contributor.examiningcommitteePierce, Grant (Physiology and Pathophysiology) Dodd, Janice (Physiology and Pathophysiology) Aliani, Michel (Human Nutritional Sciences) McLeod, Roger (Dalhousie University)en_US
dc.contributor.supervisorSiow, Chris (Physiology and Pathophysiology)en_US
dc.date.accessioned2017-09-06T20:50:39Z
dc.date.available2017-09-06T20:50:39Z
dc.date.issued2015en_US
dc.date.issued2017en_US
dc.date.issued2017en_US
dc.degree.disciplinePhysiology and Pathophysiologyen_US
dc.degree.levelDoctor of Philosophy (Ph.D.)en_US
dc.description.abstractLingonberry (Vaccinium vitis-idaea) harvested in Manitoba, Canada contains very high levels of anthocyanins and other polyphenols. These compounds are bioavailable in the kidney and, since other antioxidant compounds have been shown to protect against ischemia-reperfusion (IR) injury in various models, we hypothesized that the anthocyanins and polyphenols in Manitoba lingonberry would be bioactive against IR injury. Due to the significance of cardiomyocyte IR injury in cardiovascular disease and the prevalence of renal IR injury following major surgeries and kidney transplants, we selected three IR models to test for protective effects of Manitoba lingonberry against these types of injuries: simulated IR in H9c2 cardiomyoblasts, acute kidney injury (AKI) in Sprague-Dawley rats, and simulated IR in HK-2 kidney proximal tubule epithelial cells. First, the study showed the strong antioxidant capacity of Manitoba lingonberry and determined the major anthocyanins present. Second, the results showed that Manitoba lingonberry prevented apoptosis in H9c2 cells caused by IR injury or hydrogen peroxide-induced oxidative stress by inhibiting chromatin condensation, caspase activation, and MAPK signaling. Third, the study showed that rats who consumed lingonberry juice daily for three weeks had improved kidney function, reduced renal MAPK signaling, and an attenuated inflammatory response following AKI. Fourth, the link between renal MAPK signaling and inflammatory response was confirmed in HK-2 cells and pure lingonberry anthocyanins were shown to be effective at modulating this response. ii These results provide the first characterization of Manitoba lingonberry and highlight the importance of dietary berry compounds for cardiovascular and renal health. This study is the first to demonstrate the use of Manitoba lingonberry juice as a functional food and the first to demonstrate that physiological nanomolar doses of anthocyanins can protect heart and kidney cells from apoptosis.en_US
dc.description.noteOctober 2017en_US
dc.identifier.citationIsaak CK, Petkau JC, O K, Debnath SC, Siow YL. Manitoba lingonberry (Vaccinium vitis-idaea) bioactivities in ischemia-reperfusion injury. J Agric Food Chem 63: 5660-5669, 2015. DOI: 10.1021/acs.jafc.5b00797.en_US
dc.identifier.citationIsaak CK, Petkau JC, Blewett HJ, O K, Siow YL. Lingonberry anthocyanins portect cardiac cells from oxidative stress-induced apoptosis. Can J Physiol Pharmacol 95: 904-910, 2017a. DOI: 10.1139/cjpp-2016-0667.en_US
dc.identifier.citationIsaak CK, Wang P, Prashar S, O K, Brown DC, Debnath SC, Siow, YL. Supplementing diet with Manitoba lingonberry juice reduces kidney ischemia-reperfusion injury. J Sci Food Agric 97: 3065-3076, 2017b. DOI: 10.1002/jsfa.8200.en_US
dc.identifier.urihttp://hdl.handle.net/1993/32469
dc.language.isoengen_US
dc.publisherJournal of Agriculture and Food Chemistryen_US
dc.publisherCanadian Journal of Physiology and Pharmacologyen_US
dc.publisherJournal of the Science of Food and Agricultureen_US
dc.rightsopen accessen_US
dc.subjectLingonberryen_US
dc.subjectVacciniumen_US
dc.subjectBerriesen_US
dc.subjectAnthocyaninen_US
dc.subjectIschemiaen_US
dc.subjectReperfusionen_US
dc.subjectAcute kidney injuryen_US
dc.titleManitoba lingonberry (Vaccinium vitis-idaea) protects against ischemia-reperfusion injuryen_US
dc.typedoctoral thesisen_US
local.subject.manitobayesen_US
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