Antioxidant properties of alkaline extracts from insoluble and soluble dietary fibre derived from selected whole-grain cereals

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dc.contributor.authorGuo, Weiwei
dc.contributor.examiningcommitteeFulcher, Gary (Food Science) Netticadan, Thomas (Physiology)en_US
dc.contributor.supervisorBeta, Trust (Food Science)en_US
dc.date.accessioned2012-08-23T16:30:39Z
dc.date.available2012-08-23T16:30:39Z
dc.date.issued2012-08-23
dc.degree.disciplineFood Scienceen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractThe extraction yields of insoluble dietary fibre (IDF) and soluble dietary fibre (SDF) from seven whole-grain cereals (WG) ranged from 11.73% to 23.71% and 2.28% to 5.15%, respectively. Eight monomeric phenolic acids and four diferulic acids were identified and quantified in cereal alkaline extracts by reversed phase high performance liquid chromatography coupled with quadrupole - time of flight mass spectrometry. IDF alkaline extracts had significantly (p < 0.05) higher levels of total phenolic content (TPC) and DPPH radical scavenging activity than WG and SDF extracts. Corn IDF (C-IDF) extracts exhibited the highest TPC and DPPH, followed by red rice. MTT cell viability assay indicated that 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) or xanthine-xanthine oxidase (X-XO) induced cell loss can be effectively reduced by pre-incubating Caco-2BBe or FHs 74 Int cells with certain levels of C-IDF extracts. This effect was speculated to be associated with the antioxidant activity of C-IDF linked phenolic compounds.en_US
dc.description.noteOctober 2012en_US
dc.identifier.urihttp://hdl.handle.net/1993/8462
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectwhole-grain cerealsen_US
dc.subjectinsoluble dietary fibreen_US
dc.subjectsoluble dietary fibreen_US
dc.subjectalkaline extractsen_US
dc.subjectantioxidant activityen_US
dc.titleAntioxidant properties of alkaline extracts from insoluble and soluble dietary fibre derived from selected whole-grain cerealsen_US
dc.typemaster thesisen_US
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