Evidence for a luminal, calcium-mediated cross talk between SERCA1 pumps and RyR1 calcium release channels in heavy sarcoplasmic reticulum, HSR, membranes
| dc.contributor.author | Palahniuk, Chris G. | en_US |
| dc.date.accessioned | 2007-07-12T17:46:13Z | |
| dc.date.available | 2007-07-12T17:46:13Z | |
| dc.date.issued | 2000-06-01T00:00:00Z | en_US |
| dc.degree.discipline | Oral Biology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | The potential for luminal Ca2+ to modulate the activity of both SERCA1 pumps and RyR1 channels was examined in isolated skeletal muscle heavy sarcoplasmic reticulum vesicles. A synchronous assay was developed using Calcium Green-2 to monitor extravesicular Ca2+ and NADH oxidation as an indicator of SERCA1 activity. Results from these assays demonstrated that SERCA1 pumps responded with near-maximal activity to RyR1-mediated Ca 2+ leak states. Therefore the activities of both the SERCA1 and the RyR1 appear intertwined through a luminal, Ca2+ mediated, cross talk, such that the formation of SERCA1-mediated luminal Ca2+ gradients required for RyR1-mediated Ca2+ releases is dependent upon the distribution of Ca2+ between free and bound luminal Ca2+ compartments. (Abstract shortened by UMI.) | en_US |
| dc.format.extent | 9215980 bytes | |
| dc.format.extent | 184 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.format.mimetype | text/plain | |
| dc.identifier.uri | http://hdl.handle.net/1993/2476 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.title | Evidence for a luminal, calcium-mediated cross talk between SERCA1 pumps and RyR1 calcium release channels in heavy sarcoplasmic reticulum, HSR, membranes | en_US |
| dc.type | master thesis | en_US |