Human adenovirus (HAdV) is a critical tool and essential model for studying virology, cell biology and molecular biology. Research into HAdV has led to a comprehensive understanding of the virus and its interaction with the host cell. These properties have been used to exploit HAdV to develop therapeutics and vaccines, and to understand the cell’s inner workings. This study first explores the use of HAdV as a molecular biology tool to develop and create a set of recombinant HAdV (rHAdV) to be used as potential vaccines or research reagents for the coronavirus disease 2019 (COVID-19) pandemic. Secondly, it utilizes the critical viral protein Early 1A (E1A) to identify the cellular E1A interaction hub protein, arginine and glutamate rich 1 (ARGLU1).