Effects of canopy cover and pH on polyketide synthase gene transcription in Cladonia stygia and the polyketides produced in natural conditions.

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dc.contributor.authorChhoker, Kamaldeep
dc.contributor.examiningcommitteeWeihrauch, Dirk (Biological Sciences) Sorensen, John (Chemistry)en_US
dc.contributor.supervisorPiercey-Normore, Michele (Biological Sciences)en_US
dc.date.accessioned2018-09-18T16:13:07Z
dc.date.available2018-09-18T16:13:07Z
dc.date.issued2018-07-21en_US
dc.date.submitted2018-07-21T19:40:58Zen
dc.date.submitted2018-09-17T19:17:29Zen
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractPolyketide synthase (PKS) genes encode large multidomain enzymes that synthesize polyketides (secondary metabolites) but little is known about their biosynthesis. The goals of this study were to identify PKS genes in the Cladonia rangiferina genome in order to target PKS genes in Cladonia stygia, and to compare PKS gene transcription and polyketide production in Cladonia stygia collected from two geologically different locations in Manitoba. The Antibiotic and Secondary Metabolite Analysis Shell (antiSMASH) program identified 41 secondary metabolite gene clusters consisting of 15 Type 1 PKS genes but only two Non-Reducing Type 1 PKS clusters. The significant difference in the concentration of the polyketides, atranorin and fumarprotocetraric acid, between the two locations corresponded with gene expression using the 21 Beta-Ketoacyl Synthase (p=0.040) and 26 Thioesterase domain (p=0.081), suggesting that these PKS domains may play a role in the production of fumarprotocetraric acid and atranorin, respectively, in the C. stygia thallus.en_US
dc.description.noteOctober 2018en_US
dc.identifier.urihttp://hdl.handle.net/1993/33441
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectCladonia Stygiaen_US
dc.subjectPolyketidesen_US
dc.subjectSecondary Metabolitesen_US
dc.subjectLichenen_US
dc.subjectReindeer Lichenen_US
dc.titleEffects of canopy cover and pH on polyketide synthase gene transcription in Cladonia stygia and the polyketides produced in natural conditions.en_US
dc.typemaster thesisen_US
local.subject.manitobayesen_US
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