Development of an EBOV-GP virus entry system and investigation of the anti-EBOV activity of an extract of Prunella vulgaris and Investigation of the regulatory role of IL-34 in macrophages and CD4+ T lymphocytes during HIV-1 infection
| dc.contributor.author | Zhang, Xu | |
| dc.contributor.examiningcommittee | Drebot, Mike (Medical Microbiology and Infectious Diseases) Xie, Jiuyong (Physiology and Pathophysiology) | en_US |
| dc.contributor.supervisor | Xiaojian, Yao (Medical Microbiology and Infectious Diseases) | en_US |
| dc.date.accessioned | 2017-08-15T13:15:32Z | |
| dc.date.available | 2017-08-15T13:15:32Z | |
| dc.date.issued | 2016-02 | en_US |
| dc.date.issued | 2016-03 | en_US |
| dc.degree.discipline | Medical Microbiology and Infectious Diseases | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | In this study, we developed an EBOV-glycoprotein (GP) pseudotyped HIV-1-based vector system for the screening of anti-EBOV-GP agent(s). Based on this system, we demonstrated that an aqueous extract (CHPV) from the Chinese herb Prunella vulgaris displayed a potent inhibitory effect on EBOV-GP pseudotyped virus (EBOV-GP-V)-mediated infection in HUVECs and macrophage cell lines. In addition, our results indicated that CHPV was able to block the wild type Ebola virus infection in VeroE6 cells. The another project of my study is focused on investigation of the stimulating effect of IL-34 on HIV-1 infection in MDMs (monocytes-derived macrophages) and CD4 T lymphocytes. Our study has revealed that IL-34 as well as M-CSF does not affect the susceptibility of CD4+ T cells directly, but IL-34 or M-CSF stimulated macrophages to release some unknown soluble factors, which are able to stimulate CD4+ T cells and render them more susceptible to HIV-1 infection.In this study, we developed an EBOV-glycoprotein (GP) pseudotyped HIV-1-based vector system for the screening of anti-EBOV-GP agent(s). Based on this system, we demonstrated that an aqueous extract (CHPV) from the Chinese herb Prunella vulgaris displayed a potent inhibitory effect on EBOV-GP pseudotyped virus (EBOV-GP-V)-mediated infection in HUVECs and macrophage cell lines. In addition, our results indicated that CHPV was able to block the wild type Ebola virus infection in VeroE6 cells. The another project of my study is focused on investigation of the stimulating effect of IL-34 on HIV-1 infection in MDMs (monocytes-derived macrophages) and CD4 T lymphocytes. Our study has revealed that IL-34 as well as M-CSF does not affect the susceptibility of CD4+ T cells directly, but IL-34 or M-CSF stimulated macrophages to release some unknown soluble factors, which are able to stimulate CD4+ T cells and render them more susceptible to HIV-1 infection. | en_US |
| dc.description.note | October 2017 | en_US |
| dc.identifier.citation | Ao, Zhujun, et al. "Characterization of the single cycle replication of HIV-1 expressing Gaussia luciferase in human PBMCs, macrophages, and in CD4+ T cell-grafted nude mouse." Journal of virological methods 228 (2016): 95-102. | en_US |
| dc.identifier.citation | Zhang, Xu, et al. "Characterization of the inhibitory effect of an extract of Prunella vulgaris on Ebola virus glycoprotein (GP)-mediated virus entry and infection." Antiviral research 127 (2016): 20-31. | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/32349 | |
| dc.language.iso | eng | en_US |
| dc.publisher | Antiviral Research | en_US |
| dc.publisher | Journal of Virological Methods | en_US |
| dc.rights | open access | en_US |
| dc.subject | Ebola virus (EBOV) | en_US |
| dc.subject | Glycoprotein (GP) | en_US |
| dc.subject | Chinese herb Prunella vulgaris | en_US |
| dc.subject | Human immunodeficiency virus (HIV) | en_US |
| dc.subject | Interleukin 34 (IL-34) | en_US |
| dc.subject | Macrophage | en_US |
| dc.title | Development of an EBOV-GP virus entry system and investigation of the anti-EBOV activity of an extract of Prunella vulgaris and Investigation of the regulatory role of IL-34 in macrophages and CD4+ T lymphocytes during HIV-1 infection | en_US |
| dc.type | master thesis | en_US |