In this study, we developed an EBOV-glycoprotein (GP) pseudotyped HIV-1-based vector system for the screening of anti-EBOV-GP agent(s). Based on this system, we demonstrated that an aqueous extract (CHPV) from the Chinese herb Prunella vulgaris displayed a potent inhibitory effect on EBOV-GP pseudotyped virus (EBOV-GP-V)-mediated infection in HUVECs and macrophage cell lines. In addition, our results indicated that CHPV was able to block the wild type Ebola virus infection in VeroE6 cells. The another project of my study is focused on investigation of the stimulating effect of IL-34 on HIV-1 infection in MDMs (monocytes-derived macrophages) and CD4 T lymphocytes. Our study has revealed that IL-34 as well as M-CSF does not affect the susceptibility of CD4+ T cells directly, but IL-34 or M-CSF stimulated macrophages to release some unknown soluble factors, which are able to stimulate CD4+ T cells and render them more susceptible to HIV-1 infection.In this study, we developed an EBOV-glycoprotein (GP) pseudotyped HIV-1-based vector system for the screening of anti-EBOV-GP agent(s). Based on this system, we demonstrated that an aqueous extract (CHPV) from the Chinese herb Prunella vulgaris displayed a potent inhibitory effect on EBOV-GP pseudotyped virus (EBOV-GP-V)-mediated infection in HUVECs and macrophage cell lines. In addition, our results indicated that CHPV was able to block the wild type Ebola virus infection in VeroE6 cells. The another project of my study is focused on investigation of the stimulating effect of IL-34 on HIV-1 infection in MDMs (monocytes-derived macrophages) and CD4 T lymphocytes. Our study has revealed that IL-34 as well as M-CSF does not affect the susceptibility of CD4+ T cells directly, but IL-34 or M-CSF stimulated macrophages to release some unknown soluble factors, which are able to stimulate CD4+ T cells and render them more susceptible to HIV-1 infection.