Effects of mitogen-activated protein kinases on nuclear protein import

dc.contributor.authorFaustino, RS
dc.contributor.authorRousseau, DC
dc.contributor.authorLandry, MN
dc.contributor.authorKostenuk, AL
dc.contributor.authorPierce, GN
dc.description.abstractERK-2 MAP kinase activation induces inhibitory effects on nuclear protein import in vascular smooth muscle cells. The mechanism and characteristics of this effect of ERK-2 were investigated. An Unusual dose-dependent effect of ERK-2 on nuclear protein import was identified. At higher concentrations (1 mu g/mL) of ERK-2, nuclear protein import was stimulated. whereas lower concentrations (0.04 mu g/mL) inhibited import. Intermediate concentrations exerted intermediate effects. The stimulatory and inhibitory, effects at the 2 different ERK-2 concentrations were observed in both conventional. permeabilized cell assays of nuclear protein import and With in situ microinjection of smooth muscle cells. The biphasic effects of ERK-2 oil import were also found for the other 2 members of the MAPK family. p38 and JNK. Ran-GAP was identified by structural analysis as a candidate target protein responsible for mediating the effects of ERK-2. After pretreatment with high concentrations of ERK-2. RanGAP activity was significantly increased by similar to 50%. In contrast. low concentrations of ERK-2 significantly attenuated RanGAP activity. These results demonstrate that all 3 members of the MAPK family can alter nuclear protein import in opposite directions depending upon the concentration of ERK-2 Used. RanGAP represents the MAP kinase target whereby nuclear transport can be stimulated or inhibited.en
dc.format.extent327814 bytes
dc.identifier.citation0008-4212; CAN J PHYSIOL PHARMACOL, MAR-APR 2006, vol. 84, no. 39145, p.469 to 475.en
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dc.rightsopen accessen_US
dc.statusPeer revieweden
dc.subjectmitogen activated protein kinaseen
dc.subjectnuclear protein importen
dc.subjectcell proliferationen
dc.subjectcell growthen
dc.subjectPORE COMPLEXen
dc.subjectMAP KINASEen
dc.titleEffects of mitogen-activated protein kinases on nuclear protein importen
dc.typejournal articleen_US