Effects of mitogen-activated protein kinases on nuclear protein import

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dc.contributor.authorFaustino, RS
dc.contributor.authorRousseau, DC
dc.contributor.authorLandry, MN
dc.contributor.authorKostenuk, AL
dc.contributor.authorPierce, GN
dc.date.accessioned2007-10-09T18:05:06Z
dc.date.available2007-10-09T18:05:06Z
dc.date.issued2006-04-30
dc.description.abstractERK-2 MAP kinase activation induces inhibitory effects on nuclear protein import in vascular smooth muscle cells. The mechanism and characteristics of this effect of ERK-2 were investigated. An Unusual dose-dependent effect of ERK-2 on nuclear protein import was identified. At higher concentrations (1 mu g/mL) of ERK-2, nuclear protein import was stimulated. whereas lower concentrations (0.04 mu g/mL) inhibited import. Intermediate concentrations exerted intermediate effects. The stimulatory and inhibitory, effects at the 2 different ERK-2 concentrations were observed in both conventional. permeabilized cell assays of nuclear protein import and With in situ microinjection of smooth muscle cells. The biphasic effects of ERK-2 oil import were also found for the other 2 members of the MAPK family. p38 and JNK. Ran-GAP was identified by structural analysis as a candidate target protein responsible for mediating the effects of ERK-2. After pretreatment with high concentrations of ERK-2. RanGAP activity was significantly increased by similar to 50%. In contrast. low concentrations of ERK-2 significantly attenuated RanGAP activity. These results demonstrate that all 3 members of the MAPK family can alter nuclear protein import in opposite directions depending upon the concentration of ERK-2 Used. RanGAP represents the MAP kinase target whereby nuclear transport can be stimulated or inhibited.en
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dc.format.mimetypeapplication/pdf
dc.identifier.citation0008-4212; CAN J PHYSIOL PHARMACOL, MAR-APR 2006, vol. 84, no. 39145, p.469 to 475.en
dc.identifier.doihttp://dx.doi.org/10.1139/y05-131
dc.identifier.urihttp://hdl.handle.net/1993/2912
dc.language.isoengen_US
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dc.rightsopen accessen_US
dc.statusPeer revieweden
dc.subjectmitogen activated protein kinaseen
dc.subjectnuclear protein importen
dc.subjectcell proliferationen
dc.subjectnucleusen
dc.subjectcell growthen
dc.subjectPORE COMPLEXen
dc.subjectMAP KINASEen
dc.subjectTRANSPORTen
dc.subjectPHOSPHORYLATIONen
dc.subjectINHIBITIONen
dc.subjectPATHWAYen
dc.subjectRANGAP1en
dc.subjectRANen
dc.subjectNUCLEOPORINSen
dc.subjectINVOLVEMENTen
dc.titleEffects of mitogen-activated protein kinases on nuclear protein importen
dc.typejournal articleen_US
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