Identification of the Metabolic Enzyme Involved Morusin Metabolism and Characterization of Its Metabolites by Ultraperformance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS/MS)

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dc.contributor.authorShi, Xianbao
dc.contributor.authorMackie, Brianna
dc.contributor.authorZhang, Gang
dc.contributor.authorYang, Shuman
dc.contributor.authorSong, Yonggui
dc.contributor.authorSu, Dan
dc.contributor.authorLiu, Yali
dc.contributor.authorShan, Lina
dc.date.accessioned2016-09-20T13:57:11Z
dc.date.available2016-09-20T13:57:11Z
dc.date.issued2016-9-6
dc.date.updated2016-09-20T07:50:58Z
dc.description.abstractMorusin, the important active component of a traditional Chinese medicine, Morus alba L., has been shown to exhibit many vital pharmacological activities. In this study, six recombinant CYP450 supersomes and liver microsomes were used to perform metabolic studies. Chemical inhibition studies and screening assays with recombinant human cytochrome P450s were also used to characterize the CYP450 isoforms involved in morusin metabolism. The morusin metabolites identified varied greatly among different species. Eight metabolites of morusin were detected in the liver microsomes from pigs (PLMs), rats (RLMs), and monkeys (MLMs) by LC-MS/MS and six metabolites were detected in the liver microsomes from humans (HLMs), rabbits (RAMs), and dogs (DLMs). Four metabolites (M1, M2, M5, and M7) were found in all species and hydroxylation was the major metabolic transformation. CYP1A2, CYP2C9, CYP2D6, CYP2E1, CYP3A4, and CYP2C19 contributed differently to the metabolism of morusin. Compared to other CYP450 isoforms, CYP3A4 played the most significant role in the metabolism of morusin in human liver microsomes. These results are significant to better understand the metabolic behaviors of morusin among various species.
dc.description.versionPeer Reviewed
dc.identifier.citationXianbao Shi, Brianna Mackie, Gang Zhang, et al., “Identification of the Metabolic Enzyme Involved Morusin Metabolism and Characterization of Its Metabolites by Ultraperformance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS/MS),” Evidence-Based Complementary and Alternative Medicine, vol. 2016, Article ID 9240103, 10 pages, 2016. doi:10.1155/2016/9240103
dc.identifier.urihttp://dx.doi.org/10.1155/2016/9240103
dc.identifier.urihttp://hdl.handle.net/1993/31822
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderCopyright © 2016 Xianbao Shi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleIdentification of the Metabolic Enzyme Involved Morusin Metabolism and Characterization of Its Metabolites by Ultraperformance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS/MS)
dc.typeJournal Article
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