Raman and SERS studies of filamentous fungi

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dc.contributor.authorFarazkhorasani, Fatemeh
dc.contributor.examiningcommitteePiercey-Normore, Michele (Biological Sciences) Sorensen, John (Chemistry) van Wijngaarden, Jennifer (Chemistry)en_US
dc.contributor.supervisorGoltz, Douglas (Chemistry)en_US
dc.date.accessioned2014-08-22T16:16:06Z
dc.date.available2014-08-22T16:16:06Z
dc.date.issued2012en_US
dc.degree.disciplineChemistryen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractFungal species perform many important roles in biotechnology and recycling and act as agents of disease and decay. Surface-enhanced Raman scattering (SERS) has attracted significant attention as an analytical method for chemical and biological identification. For SERS experiments, it is essential to generate gold nanoparticles (AuNPs) with proper sizes and shapes. Raman and SERS imaging of fungi via in vivo synthesis of AuNPs were used to explore cellular components of Aspergillus nidulans (A. nidulans) cell. Critical parameters including pH, temperature and metal concentration affect the sizes and shapes of the NPs. For better control of NP formation (size, shape and location), pre-formed NP were incubated with A. nidulans colonies. Aspergillus nidulans outer hyphal walls were coated with NPs. Raman and SERS imaging of fungal walls revealed that proteins, carbohydrates and lipids are the main constituents of fungal cell wall.en_US
dc.description.noteOctober 2014en_US
dc.identifier.citationAnalyst, 2012, 37, 4934-4942en_US
dc.identifier.urihttp://hdl.handle.net/1993/23855
dc.language.isoengen_US
dc.publisherRoyal Society of Chemistryen_US
dc.rightsopen accessen_US
dc.subjectRaman spectroscopyen_US
dc.subjectSERS imagingen_US
dc.subjectnanoparticlesen_US
dc.subjectfungien_US
dc.titleRaman and SERS studies of filamentous fungien_US
dc.typemaster thesisen_US
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