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dc.contributor.supervisor Peng, Zhikang (Immunology) en_US
dc.contributor.author Moreno Martinez, Sem
dc.date.accessioned 2012-10-25T14:49:00Z
dc.date.available 2012-10-25T14:49:00Z
dc.date.issued 2012-10-25
dc.identifier.uri http://hdl.handle.net/1993/9676
dc.description.abstract Myeloid-derived suppressor cells (MDSCs), characterized by the co-expression of CD11b and Gr1, are a heterogeneous population of immature myeloid cells that exhibit strong suppressive functions against T cell responses. In inflammatory conditions like IBD, there is an increase in MDSCs but this is not sufficient to improve intestinal inflammation in IBD. Herein, we investigated the expansion of MDSCs in TNBS-induced acute colitis and whether the adoptive transfer of in vitro generated MDSCs ameliorated intestinal inflammation. We found that CD11b+Gr1+ MDSCs were significantly increased in experimental colitis. Further, this increase correlated to some extent with the severity of the disease. As per our protocol, MDSCs were generated from bone marrow cells co-cultured with hepatic stellate cells (HSCs), an essential cell type to obtain functional MDSCs in vitro. Adoptive transfer of HSC-induced MDSCs improved body weight loss and significantly downregulated inflammatory cytokines TNF, IFN-γ, and IL-17 in colonic tissue. Our results indicate MDSCs are immunoregulatory players in intestinal inflammation and that the adoptive transfer of in vitro generated MDSCs may provide a novel therapeutic approach for inflammatory bowel disease. en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject Myeloid-derived suppressor cells en_US
dc.subject Inflammatory bowel disease en_US
dc.subject Immunology en_US
dc.title Role of myeloid-derived suppressor cells in TNBS-induced murine colitis en_US
dc.type info:eu-repo/semantics/masterThesis
dc.degree.discipline Immunology en_US
dc.contributor.examiningcommittee Kung, Sam (Immunology) Warrington, Richard (Internal Medicine) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note February 2013 en_US


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