Seed quality improvement in the ogu-INRA CMS system in Oilseed Rape (Brassica napus L.)
Asselin, Sean Robert
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The ogu-INRA cytoplasmic male sterility (CMS) system is the global leader for the development of high quality hybrid canola and high erucic acid rapeseed (HEAR) cultivars of oilseed rape (Brassica napus L.). The largest challenge for plant breeders using this system is the development of high quality restorer lines (R-lines) due to tight linkage of the radish (Raphanus sativus L.) derived restorer gene PPR-B and elevated seed glucosinolate concentration. The purpose of this study was to identify improved quality restorer lines for hybrid cultivar development through both field studies and molecular marker development. In the first study 67 R-lines of different genetic backgrounds were screened over the course of two growing seasons and lines with significantly reduced glucosinolate concentration were identified. In the second study a new sequence characterized amplified region (SCAR) marker was successfully developed for the rapid screening of selections for the ogu-INRA CMS restorer gene PPR-B.