Show simple item record

dc.contributor.supervisorKirshenbaum, Lorrie (Physiology)en_US
dc.contributor.authorMughal, Wajihah
dc.date.accessioned2012-08-10T19:54:14Z
dc.date.available2012-08-10T19:54:14Z
dc.date.issued2012-08-10
dc.identifier.urihttp://hdl.handle.net/1993/8158
dc.description.abstractE2F-1 is a transcription factor that is involved in cellular growth and regulates the transition between G1 and S phase during the cell cycle. However, the mechanisms by which E2F-1 regulates endoplasmic reticulum (ER) stress in ventricular myocytes remain poorly defined. ER stress was triggered by tunicamycin or thapsigargin; gene transcription was assessed by polymerase chain reaction and protein expression was detected by western blot. Cell viability and mitochondrial defects were assessed by fluorescent microscopy imaging. During ER stress, E2F-1 repressed signaling molecules of the unfolded protein response (UPR) and sensitized myocytes to cell death triggered by thapsigargin that was inhibited in Bnip3 null fibroblasts. Bnip3Δex3 rescued thapsigargin-induced cardiac apoptosis, blocked mitochondrial defects and rescued hypoxia/ER stress induced cardiac cell death. This study provides evidence that E2F-1 sensitizes ventricular myocytes to ER stress induced apoptosis 1) by repressing the UPR; 2) that is Bnip3 dependent; and 3) mediated by mitochondrial dysfunction.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMyocytesen_US
dc.subjectEndoplasmic reticulumen_US
dc.titleRegulation of endoplasmic reticulum stress by transcription factor E2F-1 in ventricular myocytesen_US
dc.typeinfo:eu-repo/semantics/masterThesis
dc.typemaster thesisen_US
dc.degree.disciplinePhysiologyen_US
dc.contributor.examiningcommitteeSingal, Pawan (Physiology) Dhalla, NS (Physiology) Czubryt, Michael (Physiology) Duhamel, Todd (Kinesiology and Recreation Management)en_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.noteOctober 2012en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record