Measurement and characterization of HIV inhibitory Clade A Serpins in the cervical mucosa of highly HIV-1 exposed seronegative individuals

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dc.contributor.supervisor Ball, Blake (Medical Microbiology) Plummer, Frank (Medical Microbiology) en_US
dc.contributor.author Rahman, Syeda Sharmin
dc.date.accessioned 2012-01-10T21:54:32Z
dc.date.available 2012-01-10T21:54:32Z
dc.date.issued 2011-11-04
dc.identifier.citation A. Burgener et al. Comprehensive Proteomic study identifies serpins and cystatin antiproteases as novel correlates of HIV-1 resistance in the ceervicovaginal mucosa of female sex workers en_US
dc.identifier.uri http://hdl.handle.net/1993/5065
dc.description.abstract Objective: Serpins are serine protease inhibitors that are involved in a wide variety of biological functions in nature. They are known to regulate inflammation processes as well as provide host defense against microorganisms. Recent evidence has associated many types of mucosal serpins with a protective phenotype against HIV infection in women. Our hypothesis is that serpins with known antiviral activity against HIV-1 are correlated with protection in a group of HIV exposed seronegative individuals (HIV-resistant) from the Pumwani sex worker cohort. Study design: Cervico-vaginal lavage (CVL) fluid was collected from 66 HIV-positive, 82 HIV-negative and 84 HIV-resistant sex workers from the cohort. Clinical and epidemiological information was recorded at the time of sample collection. CVL protein levels were determined by BCA assay and serpin (A1 and A3) concentrations by a commercially available ELISA kit. Mucosal serpin concentrations were compared against clinical and epidemiological factors as well as sexual practices. Results: Serpin A1 was significantly higher in the HIV-resistant group compared to the HIV-negative controls (Anova: p=0.0470*). Total concentration of serpin A3 did not reach statistical significance between groups. Serpins did not correlate with age, sexual practices, contraceptive use or number of pregnancies. Serpins were differentially abundant during different stages of the menstrual cycle whereas serpin A1 was elevated during the proliferation phase but not in secretory phase (p=0.0275*). Conclusion: Serpin A1 was correlated with HIV-protection in this group of HESN women. This work will contribute to a more complete understanding of mechanisms of resistance and susceptibility to HIV infection. en_US
dc.publisher American Chemical Society (ACS) Publications en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject HIV-1 en_US
dc.subject Serpins en_US
dc.title Measurement and characterization of HIV inhibitory Clade A Serpins in the cervical mucosa of highly HIV-1 exposed seronegative individuals en_US
dc.type info:eu-repo/semantics/masterThesis
dc.type master thesis en_US
dc.degree.discipline Medical Microbiology en_US
dc.contributor.examiningcommittee Carpenter, Michael (Medical Microbiology) Yao, Xiao-Jian (Medical Microbiology) Ho, Emmanuel (Pharmacy) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note February 2012 en_US

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