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dc.contributor.supervisor Yao, Xiaojian (Medical Microbiology) en
dc.contributor.author Parvez, Md. Kamal Uddin
dc.date.accessioned 2011-04-12T17:31:06Z
dc.date.available 2011-04-12T17:31:06Z
dc.date.issued 2011-04-12T17:31:06Z
dc.identifier.uri http://hdl.handle.net/1993/4525
dc.description.abstract HIV-1 integrase (IN) enzyme employs several viral and cellular proteins for nuclear translocation and crucial integration of viral cDNA. Successful identification of new viral/cellular interactions may shed light for better understanding of HIV-1 replication. 293T cells were transiently transfected with pYEF-1-TAP-IN and cell lysate were subjected to Tandem Affinity Purification system to pull down putative IN-interacting cellular partners. A number of distinct bands from the Coomassie-stained gel were excised followed by in-gel digestion and mass spectrometry. Putative cellular partners of HIV-1 IN were heat shock protein 60 (HSP60), β-tubulin, γ-actin, ATP synthase alpha subunit and histone H1.2 were identified by mass spectrometry. Additionally, SF3A3 (splicing factor 3A3), another previously reported factor, was successfully co-immunoprecipitated with IN. The C-terminal portion of IN was found to be the region of interaction with SF3A3. Overall, this study has provided better understanding of IN dynamics enriching existing knowledge of HIV-1 IN biology. en
dc.format.extent 7332987 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject HIV-1 en
dc.subject Integrase en
dc.title Identification and characterization of new cellular interacting proteins of HIV-1 integrase en
dc.degree.discipline Medical Microbiology en
dc.contributor.examiningcommittee Fowke, Keith (Medical Microbiology) Peng, Zhikang (Immunology) Cheng, keding (Human Anatomy & Cell Science) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note May 2011 en


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