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dc.contributor.supervisor Eisenstat, David (Human Anatomy and Cell Science) en
dc.contributor.author Pinto, Vanessa Indira
dc.date.accessioned 2010-09-10T15:18:25Z
dc.date.available 2010-09-10T15:18:25Z
dc.date.issued 2010-09-10T15:18:25Z
dc.identifier.uri http://hdl.handle.net/1993/4126
dc.description.abstract DLX transcriptional targets have been implicated during retinal development. The Crx (Cone-Rod homeobox) gene is required for the differentiation and maintenance of cone and rod photoreceptors. Otx2 (Orthodenticle homeobox 2) is a key regulator of photoreceptor cell fate. The Dlx1/Dlx2 mutant mouse retina has a significant reduction of retinal ganglion cells with aberrant Crx expression in the neuroblastic layer and increased retinal Otx2 expression. We hypothesized that the Dlx homeobox genes directly repress Crx and Otx2 expression during retinal development. Expression of CRX demonstrates increased transcript and protein expression in the Dlx1/Dlx2 double knockout retina at E18.5, suggesting that these DLX transcription factors may repress CRX expression. OTX2 expression is increased in the Dlx1/Dlx2 knockout retina at E16.5 suggesting that DLX2 negatively regulates OTX2 expression. The Dlx1/Dlx2 knockout has aberrant and ectopic expression of CRX in the retina along with increased OTX2 expression. Our data suggests that both CRX and OTX2 are transcriptional targets directly repressed by the DLX1 and DLX2. en
dc.format.extent 2854738 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.subject retina en
dc.subject DLX en
dc.subject OTX2 en
dc.subject CRX en
dc.title DLX homeobox transcriptional regulation of CRX and OTX2 gene expression during vertebrate retinal development en
dc.degree.discipline Biochemistry and Medical Genetics en
dc.contributor.examiningcommittee Wigle, Jeffrey (Biochemistry and Medical Genetics) Merz, David (Biochemistry and Medical Genetics) en
dc.degree.level Master of Science (M.Sc.) en
dc.description.note October 2010 en


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