Electrospinning is a novel technique to fabricate non-woven fibers with sizes ranging from nano to micrometers. Polymers have been electrospun into nanofibers that can be developed into desirable materials with excellent biocompatibility and biodegradability for biomedical applications in wound healing and tissue regeneration. These nanofiber materials can be further functionalized to be loaded with bioactive molecules, including antibacterial agents, functional proteins that promote tissue reconstruction while protect host tissues from contamination. This study focuses on the development of multifunctional nanofibers that are incorporated with antibacterial drug(s) and immobilized with bioactive proteins. These nanofibers are potentially useful for wound care and tissue engineering scaffolding to provide both infection control and promotion of wound healing or tissue regenerations.