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dc.contributor.supervisor Kung, Sam (Immunology) en_US
dc.contributor.author Rahman, Rahmat Naureen
dc.date.accessioned 2019-03-01T19:05:01Z
dc.date.available 2019-03-01T19:05:01Z
dc.date.issued 2019-02-11 en_US
dc.date.submitted 2019-02-23T16:45:21Z en
dc.identifier.uri http://hdl.handle.net/1993/33778
dc.description.abstract JNK-associated leucine zipper protein (JLP) is a scaffold protein in the Mitogen-activated protein kinase (MAPK) known to be essential for mediating MAPK functions= such as mice spermatogenesis. The role of JLP in immune cells was unknown until recently our lab demonstrated its diverse function in the regulation of specific functions of dendritic cells (DC), B-cell, and T-cell. However, the role of JLP in the regulation of NK-cell function and/or migration remains unknown. Using a JLP-deficient (JLP-/-) mouse model, I found JLP was dispensable in the development of NK cells. IL-2 and IL-15 could activate JLP-/- NK cells in cultures. We previously demonstrated IL-2 activated NK cells showed enhanced chemotaxis towards conditioned media from LPS-mature DC in conventional trans-well assay and microfluidic devices. Thus, I tested migratory properties of IL-2 and IL-15 activated JLP+/+ (WT), JLP+/- (heterozygous) and JLP-/- (knockout) NK cells in the systems. I observed impaired migratory responses of JLP+/- and JLP-/- NK cells towards LPS conditioned medium in vitro. Our preliminary data suggested a critical role of JLP in the regulation of NK-cell migration. Future work will elucidate the underlying mechanism and other putative roles of JLP in regulating other NK-cell functions. en_US
dc.subject Immunology en_US
dc.subject Innate Immunity en_US
dc.title JNK- associated leucine zipper (JLP) scaffolding protein regulates natural killer cell migrations. en_US
dc.degree.discipline Immunology en_US
dc.contributor.examiningcommittee Uzonna, Jude (Immunology) Xie, Jiuyong (Physiology and Pathophysiology) en_US
dc.degree.level Master of Science (M.Sc.) en_US
dc.description.note May 2019 en_US


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