JNK- associated leucine zipper (JLP) scaffolding protein regulates natural killer cell migrations.
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Date
2019-02-11
Authors
Rahman, Rahmat Naureen
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Abstract
JNK-associated leucine zipper protein (JLP) is a scaffold protein in the Mitogen-activated protein kinase (MAPK) known to be essential for mediating MAPK functions= such as mice spermatogenesis. The role of JLP in immune cells was unknown until recently our lab demonstrated its diverse function in the regulation of specific functions of dendritic cells (DC), B-cell, and T-cell. However, the role of JLP in the regulation of NK-cell function and/or migration remains unknown. Using a JLP-deficient (JLP-/-) mouse model, I found JLP was dispensable in the development of NK cells. IL-2 and IL-15 could activate JLP-/- NK cells in cultures. We previously demonstrated IL-2 activated NK cells showed enhanced chemotaxis towards conditioned media from LPS-mature DC in conventional trans-well assay and microfluidic devices. Thus, I tested migratory properties of IL-2 and IL-15 activated JLP+/+ (WT), JLP+/- (heterozygous) and JLP-/- (knockout) NK cells in the systems. I observed impaired migratory responses of JLP+/- and JLP-/- NK cells towards LPS conditioned medium in vitro. Our preliminary data suggested a critical role of JLP in the regulation of NK-cell migration. Future work will elucidate the underlying mechanism and other putative roles of JLP in regulating other NK-cell functions.
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Immunology, Innate Immunity