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dc.contributor.supervisor Belmonte, Mark (Bioloical Sciences), de Kievit, Teresa (Microbiology) en_US
dc.contributor.author Becker, Michael
dc.date.accessioned 2019-01-08T15:45:17Z
dc.date.available 2019-01-08T15:45:17Z
dc.date.issued 2019-01-03 en_US
dc.date.submitted 2019-01-03T21:48:31Z en
dc.identifier.uri http://hdl.handle.net/1993/33644
dc.description.abstract Canola (Brassica napus L.) is one of the world’s most valuable oilseed crops and sustains a multibillion-dollar industry. Currently, blackleg disease caused by the hemibiotrophic fungus L. maculans threatens the canola industry in Canada, Europe, and Australia and causes over $500 Million in crop loss per annum. Disease control relies heavily on crop resistance mediated by SOBIR1-interacting receptor-like proteins, such as Rlm2 and LepR1. Understanding the downstream plant immune responses activated by SOBIR1-RLP complexes is currently an area of interest in plant pathology. This work profiles the transcriptome of canola cotyledons from the initial stages of infection through to the necrotrophic stage of disease, in both compatible and incompatible (Rlm2, LepR1) interactions. A spatial dimension was added to this dataset through the application of laser microdissection and captured early signaling events during initial host colonization. Through transcriptomic interrogation I identified plant immune responses associated with resistance, such as jasmonic acid signaling, lignin and callose production, and calcium signaling. Additionally, I designed the program SeqEnrich to build regulatory networks and predict transcriptional control of these important immune processes. SeqEnrich has been made publicly available and will serve as a valuable resource to researchers studying transcriptional control of biological processes in B. napus and Arabidopsis. Further, sequencing data was functionally validated with mutant screens, microscopy, measurement of endogenous jasmonic acid concentrations, and physiological experiments with calcium channel blockers. This identified positive regulators of plant resistance, including uncharacterized receptor-like proteins, that are the focus of ongoing research. To my knowledge, this is the first study to apply LMD and RNA-Seq in combination to characterize early signaling events during a plant host-microbe interaction, and the first transcriptomic investigation into LepR1- and Rlm2-mediated immunity. Together, this information will be valuable to researchers studying blackleg disease, regulation of transcription in plants, and plant host pathogen interactions in general. en_US
dc.rights info:eu-repo/semantics/openAccess
dc.subject Blackleg, Canola, Transcriptomics, RNA-seq, Laser microdissection, Resistance, Rlm2, LepR1 en_US
dc.title Transcriptomic characterization of the B. napus – L. maculans pathosystem en_US
dc.type info:eu-repo/semantics/doctoralThesis
dc.type doctoral thesis en_US
dc.degree.discipline Biological Sciences en_US
dc.contributor.examiningcommittee Whyard, Steven (Biological Sciences) Fernando, Dilantha (Plant Science) Strelkov, Stephen (University of Alberta) en_US
dc.degree.level Doctor of Philosophy (Ph.D.) en_US
dc.description.note February 2019 en_US


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