Development of a phage cocktail to control the top 7 shiga -toxin E. coli serogroups on fresh produce
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Date
2018
Authors
Yiran, Ding
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Abstract
Introduction: The big seven Shiga- toxigenic Escherichia coli (STEC) strains, which includes serogroups O26, O45, O111, O103, O121, O145, and O157, are recognized as a major cause of enteric disease worldwide.
Purpose: To evaluated the effectiveness of seven bacteriophages, to reduce seven E. coli serotypes on fresh produce and mung beans (MB).
Methods: Lettuce, sprouts, and MB were immersed in a cocktail of the top seven STEC strains (10^4CFU/g). Samples were treated with chlorinated water, STEC lytic phage cocktail, or a combination of both treatments. STEC survival were assessed using total plate count method on MacConkey and Rainbow agars.
Results: Phages were able to reduce STEC O26, O45, O103, O111, O121, O145, and O157 serogroups alone, or in combination with chlorinated water on lettuce, sprouts, and MB seeds without the development of phage resistant mutants. However, the STEC phage cocktail was not effective at controlling STEC populations during MB germination.
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Keywords
Bacteriophage, Fresh produce, Big six STEC