Three dimensional telomeric profiles in circulating tumour cells as a method of monitoring treatment response in high-risk prostate cancer patients
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Date
2016
Authors
Wark, Landon
Wark, Landon
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Abstract
Prostate cancer is the second most commonly diagnosed cancer in men worldwide. Because prognosis can vary depending on tumour stage, precise diagnosis is vital.
Circulating tumour cells (CTC) detach from primary and secondary tumour sites into the bloodstream.
Changes in three-dimensional (3D) nuclear organization are associated with different types of cancer and were examined in this study in CTCs of high-risk prostate cancer patients.
CTCs were isolated from 3mL of patient blood samples of 20 high-risk prostate cancer patients before treatment; after neoadjuvant androgen deprivation therapy (ADT) but before radiotherapy (RT); and after completing RT. Telomere-specific fluorescence in situ hybridization was performed on filters containing cells, 3D images of 30 CTCs per filter were analysed.
Changes in telomere organization were observed post ADT and RT; patients fell into three groups depending on the change in CTC telomeric profiles in response to ADT. These groups displayed responses characteristic to each group upon delivery of RT.
3D nuclear telomere profiles in CTCs post-ADT may indicate both ADT response and predict RT response in high-risk prostate cancer.
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Keywords
Prostate cancer, Circulating tumour cells, Telomeres